急性白血病患者骨髓内皮祖细胞的变化与微血管密度的关系

Changes of Endothelial Progenitor Cells in Bone Marrow of Acute Leukemia Patients and Its Association with Microvessel Density

目的探讨急性白血病患者骨髓内皮祖细胞(EPCs)数量变化与骨髓微血管密度的关系.方法采用流式细胞术检测36例初诊急性白血病患者骨髓EPCs数量,同时应用免疫组织化学法(SP法)检测骨髓活检样本微血管密度(MVD),以良性血液病患者10例为对照组.结果 AL患者骨髓EPCs绝对计数(108.36±15.43)个/μL较对照组(16.10±5.26)个/μL显著升高(P<0.01).AL患者骨髓MVD为(19.31±2.29)/400倍视野)较对照组(7.70±1.64)/400倍视野,显著升高(P<0.01).经Pearson相关性分析,AL患者骨髓EPCs绝对计数与骨髓MVD呈正线性相关r=0.963,P<0.01.结论内皮祖细胞在AL骨髓血管新生中可能具有一定作用,有可能成为AL抗血管生成治疗的新靶点.

Objective To evaluate the count of endothelial progenitor cells （EPCs） in bone marrow of acute leukemia （AL） patients and its association with microvessel density. Methods EPCs were quantified by flow cytometry procedures in 36 AL patients at the time of diagnosis. Microvessel density （MVD） in bone marrow was determined by immunohistochemistry method with 10 benign hemopathy patients as control group.Results Absolute count of EPCs in bone marrow of AL patients （ 108.36 ± 15.43/μL） was significantly higher than control group （16.10 ±5.26/μL） （P 〈 0.01） Compared with the control group （7.70 ± 1.64/400 magnification） , MVD in borrow marrow of acute leukemia patient （19.31 ± 2.29/400magnification） was significantly higher （P 〈 0.01） Absolute count of EPCs in bone marrow of AL patients was positively correlated with MVD value （r = 0.963, P 〈 0.01） by Pearson correlation analysis. Conclusion EPCs may play an important role in the mierovessel hyperplasia in AL, and could be a new target for the treatment of AL.