噬菌体生物扩增技术在结核分枝杆菌利福平耐药性检测中的应用

Application of the Phage Amplifted Biologically Assay in Drug Resistance Testing of Rifampcin of Mycobacterium Tuberculosis

目的评价噬菌体生物扩增技术(PhaB法)在结核分枝杆菌(MTB)利福平(RFP)耐药性检测中的应用价值.方法采用PhaB法检测临床78例肺结核患者痰标本中MTB对利福平的耐药性,并与比例法比较.结果以比例法为评价标准,PhaB法利福平耐药性检测的灵敏度、特异性、准确度分别为84.0%、94.33%、91.03%.结论 PhaB法检测痰标本中结核分枝杆菌对利福平的耐药性具有较高的敏感性和特异性,与比例法符合率高,且简便快速,不需要特殊仪器设备,可作为MTB耐药性检测的快速筛选方法.

Objective To evaluate the clinic application value of Phage amplifted biologically assay （PhaB） in drug resistance testing of Rifampcin of Mycobacterium tuberculosis. Method The sputum samples Mycobacterium tubercuolosis in 78 cases was detected by PhaB method about the drug resistance of Rifampcin and compared with the results of concentration ratio （CR） method＇s. Result CR method as a standard of criterion , the sensitivity, specitivity and accuracy of PhaB 91.03%, respectively. Conclusion Compared method in testing Rifampcin resistance was 84.0%, 94.33% and with CR methods, PhaB method has higher sensitivity andspecitivity, so it should be as a better choice for detecting drug resistance of Mycobacterium tuberculosis as it is not only simple but also rapid and without requiring sophisticated facilities.