脂肪来源干细胞在小鼠放射性损伤涎腺组织中归巢的实验研究

Homing of adipose-derived stem cells in the radiation-damaged salivary gland tissue of mice

目的:观察脂肪来源干细胞(adipose-derived stem cells,ASCs)在C57小鼠放射性损伤涎腺组织中的归巢现象。方法:体外培养C57小鼠ASCs;建立C57小鼠涎腺放射损伤模型;荧光染料DiI标记的普通C57小鼠ASCs及GFP转基因C57小鼠ASCs、分别以每次2×105/ml从尾静脉注射至涎腺放射损伤模型小鼠体内,注射3次,间隔6 h,末次注射24 h后处死小鼠,将下颌下腺制作成冷冻切片,荧光显微镜观察。结果:建立了C57小鼠ASCs细胞系及小鼠放射性涎腺损伤动物模型。尾静脉注射ASCs后在小鼠放射性损伤涎腺组织中观察到标记细胞。结论:ASCs能够归巢到小鼠放射性损伤涎腺组织中。

Objective： To investigate adipose-derived stem cell（ASCs） homing in radioactive damaged salivary gland of mice. Methods ： ASCs of C57 mice were cultured ill vitro. Tile models of C57 miee with localized radiation damaged salivary gland were es- tablished. ASCs labeled by DiI fluorescent dye and ASCs of GFP transgenic mice were respectively injected into /he C57 mice with localized radioative damaged salivary gland through caudal vein by 2× 10^5cells/ml for 3 times with the interal of 6 h. 24 h ＇after last injection the mice were killed. Frozen sections of the submandibular gland were prepaired and observed under fluorescence micro- scope. Results： The ASCs cell line of C57 mice and the model of C57 mice with localized radioative damaged salivary, gland were successflllly established, labeled cells were observed after ASCs had been injected into the C57 mice with localized radioative damaged salivary gland. Conclusion： ASCs have homing capacity to the salivary gland tissue with radiative injury in C57 miee.