改良HBSS液对体外培养人牙周膜细胞增殖活性的影响

Effect of modified HBSS on the proliferation of human periodontal ligament cells in vitro

目的:观察改良HBSS液对体外培养人牙周膜细胞(PDLCs)增殖活性的影响。方法:在添加青霉素、链霉素各100U/ml、地塞米松8 mg/L的HBSS液中分别加入1、3和5 mmol/L的还原型谷胱甘肽(GSH)制成改良HBSS液,并与HBSS液、HC-A离体肾保存液及蒸馏水比较,用MTS法测定培养第4代PDLCs在上述溶液中保存不同时间后的增殖活性。结果:HBSS液组和改良HBSS液组在0.5～48h的PDLCs增殖活性显著高于HC-A液组和蒸馏水组(后两者之间没有显著差异),而改良HBSS液含3 mmol/L GSH组和5 mmol/L GSH组在2～12 h显著高于HBSS液组,1 mmol/L GSH组在2～6 h显著高于HBSS液组,5 mmol/L GSH组在2～12 h显著高于含GSH的其它浓度组。结论:改良HBSS液保存人PDLCs增殖活性的效果好于HBSS液,其中含5 mmol/L GSH的改良液在12 h内的效果最好。

Objective： To study the effectiveness of modified Hankg balanced salt solution （HBSS） on the proliferation of human periodontal ligament cells （PDLCs） in vitro at room temperature. Methods： Human PDLCs were cultured in vitro. The modified HB- SS was prepared by adding 1 mmol/L,3 mmol/L or 5 mmol/L glutathione（GSH） into the HBSS in which 8 mg/L dexamethasone, penicillin and streptomycin at 100 U/ml of each were included. The cells of passage 4 were cultured in the modified HBSS, HC-A kidney preservation solution （HC-A） and distilled water（ H2 O） for 0. 5 ,1,2 ,6 ,12 ,24 and 48 h respectively. The proliferation of the cells was examined by MTS assay. Results ： From 0.5- 48 h, the proliferation activity of PDLCs in HBSS and modified HBSS was significantly higher than in HC- A and H2O （ HC - A vs H2O, P 〉 0.05 ）, that in 3 mmol/L GSH and 5 mmol/L GSH was significant- ly higher than in HBSS from 2 - 12 h, in 1 mmol/L GSH was significantly higher than in HBSS from 2 - 6 h, and in 5 mmol/L GSH was significantly higher than in other media containing GSH from 2 - 12 h. Conclusion： Adding GSH into HBSS can improve the ef- fectiveness for human PDLC proliferation, modified HBSS with 5 mmol/L GSH is suitable for 12 hour human PDLCs storage.