摘要
目的观察变异链球菌clpP基因缺陷株的生长特点;比较标准株与clpP基因缺陷株在氧化应激条件下的生长情况的差异。方法将两菌株分别培养在普通TPY液体培养基和含有H2O2终浓度0.0 005%的TPY培养基中,37℃微需氧条件下培养,从接种入培养基开始以2 h间隔采用酶联免疫检测仪在600 nm下测定各菌株的生长曲线并比较其生长情况的差异。结果①缺陷株在TPY液体培养基中生长时,生长周期发生改变,对数期峰值提前且此阶段生长速度较标准株快(P<0.05),而标准株的对数期峰值及稳定期A值均明显高于缺陷株(P<0.05);②标准株和缺陷株在含有H2O2的TPY液体培养基中生长时生长能力较无H2O2条件下明显降低(P<0.05)。结论 clpP基因的失活一定程度上导致变异链球菌生长能力和氧化应激能力的降低。
Objective To observe the growth characteristics of clpP mutant strains of Streptococcus mutants and to compare the growth difference of standard strains and clpP mutant strains under oxidative stress.Methods The standard strains and mutant strains were cultured respectively in TPY medium and TPY medium containing H2O2 with the terminal concentration of 0.0005% under microaerophili condition at 37℃.After inoculated in the mediums,the growth curve of each stain was determined and compared by Thermo Labsystems at an interval of 2 hours under 600nm.Results ①Growth cycle of the mutant strains changed in TPY medium.The exponential phase was ahead of time and the mutant strains grew faster than standard strains（P＆lt;0.05）.The peak value in exponential phase and A value in stationary phase of standard strains were higher than those of mutant strains（P＆lt;0.05）.②Both strains grew slower in TPY medium containing H2O2.Conclusions Inactivation of clpP gene can reduce the capacity of Streptococcu mutans to grow and to resist oxidative stress.
出处
《口腔医学》
CAS
2013年第5期315-318,共4页
Stomatology
