黄荆子乙酸乙酯提取物对人绒毛膜癌JEG-3细胞增殖与凋亡的影响及其作用机制

Proliferation and apoptosis of choriocarcinoma cell JEG-3 induced by VB2 and its in vitro mechanism

目的:观察黄荆子乙酸乙酯提取物(purified vitexin compound 2,VB2)对人类绒毛膜癌JEG-3细胞体外增殖及凋亡活性,及对凋亡相关基因mTOR/4E-BP1 mRNA表达的影响。方法:应用四甲基偶氮唑蓝(MTT)法检测不同浓度的VB2对JEG-3细胞的体外增殖活性;Hoechst 33258染色观察细胞形态学变化;流式细胞分析方法定量检测不同浓度药物作用后细胞凋亡率;RT-PCR法检测mTOR/4E-BP1 mRNA水平表达的变化。结果:1)2.5,5.0,10.0,20.0,40.0,80.0,160.0μmol/L的VB2分别作用JEG-3细胞(24,48,72 h)后,其生长抑制率由(6.34±0.41)%增加至(85.89±0.81)%,且与剂量及作用时间呈明显正相关(P<0.05)。2)0,5.0,10.0,20.0μmo l/L的VB2处理JEG-3细胞48 h后,加药组细胞有明显的凋亡形态特征;其凋亡率由(9.26±1.02)%增至(35.55±1.24)%,并呈明显剂量依赖性(P<0.05);mTOR及4EBP1 mRNA表达水平随着VB2作用浓度增高逐渐降低,二者呈负相关(P<0.05)。结论:VB2能抑制人绒毛膜癌JEG-3细胞增殖,诱导其凋亡,此作用可能与VB2抑制JEG-3细胞mTOR和4E-BP1mRNA的表达有关。

Objective： To investigate the effect of purified vitexin compound 2 （VB2）, a noval lignanoid from the acetoacetate extract of Vitex negundo seed on the proliferation and apoptosis as well as the expression of mTOR and 4E-BPI mRNA signal pathway in human choriocarcinoma JEG-3 cell lines in vitro. Methods： Tae inhibitory effect of different concentrations of VB2 on JEG-3 cells was examined by methyl thiazolyl tetrazolium （MTT） assay. Flow after using different concentrations of VB2, and the determined by RT-PCR. cytormetry was used to analyze the apoptosis expression of roTOR and 4E-BP1 mRNA was