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酒精暴露对视网膜致畸作用和细胞凋亡的影响

Prenatal alcohol exposure induces retinal teratogeny and cell apoptosis
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摘要 目的探讨产前酒精暴露(PAE)对视网膜致畸作用和细胞凋亡的影响。方法利用C57BL/6J小鼠建立孕期酒精暴露模型,用HE染色和免疫荧光染色技术观察酒精对生后0d(P0)、P7、P14和P30 4个年龄点共72只子鼠的视网膜致畸性和细胞凋亡的影响。结果高剂量酒精组中视网膜的畸形率增加;在各剂量组Caspase-3、Caspase-8、Caspase-9阳性细胞于内颗粒层中的表达与在节细胞层中的表达具有一致性,且具有剂量依赖性(P<0.05);TUNEL染色结果发现,与对照组相比,酒精组各年龄点的内颗粒层和节细胞层中细胞凋亡量均增加(P<0.05),提示孕期酒精暴露诱导视网膜细胞发生凋亡时具有长时程效应。结论视网膜畸形及细胞凋亡可能是导致PAE相关眼病的病理学基础。 Objective To investigate the effect of teratogeny and cell apoptosis on retina after prenatal alcohol exposure （PAE）. Methods A total of 72 mouse models of prenata; alcohol exposure were made. HE staining and immunofluorescent labeling were carried out to visualize the retinal pathology and cell apoptosis. Results High prenatal alcohol exposure increased the rate of retinal malformation; The expression of positive cells in inner granular layer（INL） was costistent with ganglial cell layer（GCL） in the retina with dose-dependency （P 〈 0. 05 ） ; TUNEL showed in the retina INL and GCL, alcohol induced the cell apoptosis with long-range effects （P 〈 0. 05 ）. Conclusion Retinal malformation and cell apoptosis probably are the cause of eye diseases induced by PAE.
作者 李瑞玲 周洁 王强 刘彬 黄霞 马战友 邓锦波
机构地区 河南大学护理学院神经生物学研究所
出处 《解剖学报》 CAS CSCD 北大核心 2013年第3期297-303,共7页 Acta Anatomica Sinica
基金 国家自然科学基金资助项目(30771140 31070952)
关键词 产前酒精暴露 视网膜细胞 发育 HE染色 免疫荧光染色 小鼠 Prenatal alcohol exposure Retinal cell Development HE staining Immunofluorescent labeling Mouse
分类号 R774.1 [医药卫生—眼科]
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参考文献18

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