生殖细胞核因子及Oct-4在P19细胞系诱导分化中的表达

Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line

目的体外培养未成熟畸胎瘤细胞(P 19细胞)并诱导分化,检测生殖细胞核因子(GCNF)及Oct-4在P 19细胞诱导分化前后的表达。方法用全反式维甲酸(ATRA)诱导P 19细胞分化,通过间接免疫荧光染色和RT-PCR方法检测GCNF及Oct-4在P 19细胞诱导分化前后的表达。结果 Oct-4在P 19细胞诱导前呈强阳性表达,诱导2 d后表达下降;而GCNF在P 19细胞诱导前呈阴性表达,诱导2 d后呈阳性表达。结论 GCNF参与了恶性生殖细胞肿瘤的体外分化。GCNF可能是通过下调Oct-4基因的表达参与了恶性生殖细胞肿瘤的分化。

Objective To detect the expressions of Oct-4 and germ cell nuclear factors（GCNF） before and after inducing P19 cells to differentiation. Methods The P19 cells were induced to differentiation with ATRA treatment. The immunofluoreseence staining and RT-PCR techniques were used to detect the expression of GCNF and Oct-4 before and after differentiation of mouse P19. Results The intensities for Oct-4 immunofluorescence positive staining were stronger in non-differentiated P19 cells than that in the differentiated P 19 cells. The intensities for GCNF immunofluorescence positive staining were weaker in non-differentiate P19 cells than that in the differentiated P19 cells. Conclusion GCNF is involved in the in vitro differentiation progress of the malignant germ cell tumors. GCNF be inhibited through the Oct-4 gene expression in vitro differentiation of P19 cells.