中药复方对麻黄素成瘾小鼠肾抗氧化酶和特异蛋白的影响

Effect of Chinese traditional medicine on activities of antioxidant enzyme and special protein of kidney in ephedrine addiction mice

目的探讨中草药对麻黄素成瘾动物的影响。方法采用递增剂量腹腔连续注射麻黄素溶液(2.0g/L、3.0g/L和4.0g/L)15d,建立麻黄素依赖动物模型,再分别给予递增剂量(20.0g/L、30.0g/L、40.0g/L)的黄芪、川芎及中药复方液进行灌胃,分别于灌胃中药5、10、15d用分光光度法检测肾组织超氧化物岐化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA))含量和血清尿素氮含量,用免疫组织化学法检测肾组织中Bax蛋白和转化生长因子-β1(TGF-β1)的表达,用生物显微技术观察肾组织结构的变化。结果实验对照组小鼠肾组织SOD和CAT活性降低,MDA含量升高,肾组织Bax蛋白和TGF-β1的阳性表达增强,血清中尿素氮含量升高,肾组织严重受损;黄芪组、川芎组及中药复方组小鼠肾组织MDA含量又降低(P<0.05或P<0.01),SOD和CAT活性又升高(P<0.05或P<0.01),血清中尿素氮含量降低,肾组织损伤变性明显减轻,肾组织Bax蛋白和TGF-β1的阳性表达强度减弱。结论黄芪、川芎及中药复方可提高肾组织细胞的抗氧化能力,促进组织细胞损伤修复。

Objective To research the effect of Chinese traditional medicine on ephedrine addiction mice. Methods A model of addiction in ephedrine dependent mice was established by intraperitonealy injected escalation doses （ 2.0, 3.0, 4.0 g/L ） of ephedrine 15 days,and then for withdrawal by irrigate to stomach with escalation doses（20. 0, 30. 0,40. 0g/L） astragalus and ligustici chuanxiong Hort decoction and Chinese medicine compound recipe, The samples on withdrawal at 5,10 and 15 days. The content of bloodurea nitrogen （BUN） and malonaldehyde （ MDA ） , and activities of superoxide dismutase（SOD） and catalase（CAT） were detected by spectrophotometer. The expression of Bax protein and TGF-β1 were examined by immunohistochemical methods. Structures of and the kidney were observed by optical microscopy after irrigation of Chinese medicine. Results SOD and CAT activities the of experimental control group were lower than natural control group. While MDA content increased, the expression intensity of Bax protein and TGF-β1 of the experimental control group were higher than the natural control group. The content of blood serum BUN increased structures of the kidney appeared severity harm. The SOD and CAT activity of kidney in astragalus,ligusticum chuanxiong Hort and Chinese medicine compound group increased, while the content of MDA decrease. The degeneration of kidney tissue structure significantly decreased in astragalus, ligusticum chuanxiong Hort and Chinese traditional medicine compound groups. The intensity of positive expression of Bax protein and TGF-β1 intensity decreased,The content of blood serum BUN markedly reduced. Conclusion Astragalus,ligusticum chuanxiong Hort and Chinese medicine compound recipe may improve the ability of cell anti-oxidation, promote the cell repairing and reduce cell damage.