摘要
目的探讨2型糖尿病大鼠肾小管细胞聚核糖性死亡的形态学特点。方法 20只雄性SD大鼠分为正常组和2型糖尿病组,在光学显微镜和电子显微镜下观察两组大鼠肾小管细胞结构的变化,用免疫组织化学染色、免疫印迹方法检测多聚二磷酸腺苷核糖聚合酶-1(PARP-1)蛋白的表达。结果 2型糖尿病早期肾小管细胞内有大量糖原聚集,细胞肿胀,部分细胞坏死,主要为空泡样坏死。免疫组织化学染色显示,2型糖尿病肾小管上皮细胞PARP-1表达阳性。免疫印迹法显示2型糖尿病肾小管细胞中PARP-1蛋白表达增强。结论 2型糖尿病早期肾小管细胞发生了坏死,聚核糖性死亡(PARP-1介导的细胞死亡)参与了2型糖尿病早期肾小管细胞的损伤。
Objective To observe the parthanatos involved in tubular cell damages of kidney in rats with type 2 diabetics. Methods Animal models of type 2 diabetics were established. Light microscopy, electron microscopy, immunohistochemistry and Western blotting were used in this study. Results A large number of glycogen accumulation, cell swelling, and partial necrosis, mainly for the vacuolar necrosis were seen in early type 2 diabetes renal tubular cells. Immunohistochemical staining of PARP-1 showed that the positive cells were found in epithelium of renal tubules of type 2 diabetic kidney. Compared to the control kidney, PARP-1 expressed much stronger by immunoblot analysis. Conclusion Necrosis and parthanatos are involved in renal tubule damages induced by type 2 diabetes in rats.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第3期409-412,共4页
Acta Anatomica Sinica
基金
辽宁省科技厅计划资助项目(2012225019)
