健脾益肾、活血化瘀中药复方对高糖刺激下肾小球系膜细胞增殖的影响

Effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cells proliferation in high glucose

目的探讨健脾益肾、活血化瘀中药复方对高糖刺激下大鼠肾小球系膜细胞(RMCs)增殖的影响及对转化生长因子-β1(TGF-β1)和骨形态发生蛋白-7(BMP-7)表达的影响。方法将常规培养的RMCs分为对照组(5.6 mmol/L葡萄糖组)、甘露醇组(5.6 mmol/L葡萄糖+24.4 mmol/L甘露醇)、高糖组(30 mmol/L葡萄糖组)、高糖+中药复方组(30 mmol/L葡萄糖+0.65 mg/mL中药复方)。培养48 h,采用4-甲偶氮唑蓝(MTT)法检测细胞增殖情况,分析健脾益肾、活血化瘀中药复方对高糖刺激下RMCs增殖的影响。酶联免疫法检测TGF-β1的表达,实时定量PCR法检测BMP-7 mRNA的表达。结果高糖组RMCs增生及TGF-β1的表达较对照组增加明显,BMP-7 mRNA的表达则明显下降,高糖+中药复方组较高糖组RMCs增生及TGF-β1的表达明显下降,BMP-7mRNA的表达明显升高。结论健脾益肾、活血化瘀中药复方能够抑制高糖刺激下的RMCs增殖及TGF-β1的表达,并上调BMP-7 mRNA的表达。

Objective To study the effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cell proliferation and expressions of TGF-[31 and BMP-7 in high glucose. Methods RMCs of conventional culture were divided into the normal control group, mannitol group, high glucose group and high glucose with 0.65 mg/mL traditional Chinese medicine group. The glucose concentration of normal group was 5.6 mmol/L while 30 mmol/L in the high glucose group. After 48 h, cell proliferation was detected with 4 - A thiazolyl blue. TGF-β1 was detected with ELISA. BMP-7 mRNA was detected with RT-PCR. Results Compared with the blank control group, cell proliferation and expression of TGF-131 of high glucose group were obviously increased while the level of BMP-7 mRNA was decreased. Compared with the high glucose group, cell proliferation and expression of TGF-β1 of Chinese herbal com- pound prescription obviously decreased while expression of BMP-7 mRNA was increased. Conclusion High glucose could promote the proliferation and expression of TGF-β1 of RMC. It also could decrease the expression of BMP-7 mRNA. Chinese herbal compound prescription could restrain the proliferation and expression of TGF-β1 of RMC in high glucose while it could increase the expression of BMP-7 mRNA.