猪脱细胞基质矿化支架材料的生物相容性及降解特性

Biocompatibility and biodegradation of a novel porcine acellular dermal matrix hydroxyapatited scaffold

目的探讨猪脱细胞基质矿化支架材料的生物相容性及体外降解特性。方法体内实验将猪脱细胞基质矿化支架材料(HA-PADM)、猪脱细胞基质支架材料(PADM)、明胶海绵分别埋植在12只新西兰大白兔脊柱两侧肌肉内,并且于观察时间(7、14、21、28 d)取出,组织病理学切片HE染色后观察组织的炎症反应,同时于术前、植入第2天、第4天兔耳缘静脉抽血行血常规检查;体外实验将HA-PADM、PADM、明胶海绵浸泡于细胞培养液(DMEM)中,于各个时间点(7、14、21、28 d)称重研究材料的体外降解情况。结果各时间点实验动物的血常规检测结果无显著性差异(P>0.05);三种支架材料周围肌肉组织无脓肿形成和组织坏死发生;此种支架材料的降解特性符合牙周组织工程的要求。结论猪脱细胞基质矿化支架材料生物相容性良好,降解时间适宜,是一种应用前景广阔的牙周组织工程支架材料。

Objective To investigate the biocompatibility and biodegradation of a novel porcine acellular dermal matrix hydroxyapatited （HA-PADM） scaffold. Methods The biocompatibility of three kinds of scaffolds （HA-PADM, PADM, Gelfoam） in vivo was assessed by implanting them into the sacrospinal muscle of 12 New Zealand white rabbits and extracting them after 7, 14, 21, and 28 days. Haematoxylin Eosin （HE） staining was used to evaluate the biocompatibility of the scaffolds. Blood routine examinations were carried out before the operation and 2, 4 days after the operation. The biodegradation patterns of the scaffolds were evaluated in vitro by weight assessment at different time points after cultivation in the cell-culture medium （DMEM）. Results There were no statistically differences in the results of blood routine examinations of all animals （ P 〉 0.05 ）. Around the embedded scaffolds there was no formation of abscess and no tissue necrosis of the muscle. The local tissue reaction was abacterial inflammatory response. The biodeg- radation of the scaffolds met the needs of periodontal tissue regeneration. Conclusion The biocompatibility of HA- PADM is favorable and the biodegradation time is proper. HA-PADM scaffold might be a promising scaffold for periodontal tissue regeneration.