尿激酶3'-UTR基因多态性与特发性草酸钙结石易感性的关系

Association of urokinase gene 3′-UTR ploymorphism with diopathic calcium oxalate stone

目的探讨尿激酶3'-UTR基因多态性与特发性草酸钙结石易感性的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,检测138例特发性草酸钙结石患者和142例健康对照组人群尿激酶3'-UTR基因+4506位点C/T基因多态性的表达。结果特发性草酸钙结石组与健康对照组比较,TT与CC+TC基因型频率、T/C等位基因频率进行卡方检验,差异均有统计学意义(P<0.05)。T等位基因携带者是特发性草酸钙结石的危险因素。与等位基因C相比,T基因携带者的患病风险是其1.493倍。结论尿激酶3'-UTR+4506 C/T基因多态性与特发性草酸钙结石存在相关性。

Objective To explore the association between urokinase gene 3＇- UTR T/C polymorphism and idiopathic calcium oxalate urolithiasis. Methods 142 healthy people without history of stone formation and 138 people with idiopathic calcium oxalate stones were engaged in this study. Urokinase gene 3＇-UTR T/C polymorphism was determined by polymerase chain reaction and restriction fraction length polymorphism（PCR-RP-LP）. Results The difference of TT and CT ＋ CC genotype frequencies between the two groups was significant（ P 〈 0.05 ） . The difference of T and C allele frequencies between the two groups was also significant（P 〈 0.05 ）. Allele T was an risk factor for the idiopathic calcium oxalate nephrolithiasis. The idiopathic calcium oxalate nephrolithiasis incidence risk of the T gene carrier was 1. 493 times higher compared with allele C. Conclusion There is significant association between urokinase gene 3＇- UTR T/ C polymorphism with idiopathic calcium oxalate urolithiasis.