摘要
应用重组人瘦素(Leptin,LPT),自行制备兔抗人LPT抗血清,采用Iodogen法放射性核素标记物125I标记LPT,建立了人血清LPT的放射免疫分析(Radioimmunoassay,RIA)方法。研究结果表明,该法的特异性强,灵敏度高,重复好,该法与LINCO RIA kit比较有显著相关性。检测范围:0.5~100ng/ml。批内误差CV6.97%,批间误差CV6.22%。
Human leptin antihserum was prepared in our laboratory by immunizing rabbits with purified re- combinant human leptin while 125I - leptin was obrained by iodogen method. A method of RIA for human leptin was developed, using these self - made preparations. The result of tests showed high sensitivity and specificity with reliable reproducibility, which proved that the quality of our preparations was no less good than that of the imported LINCO kit. The detectabble range was between 0.5 - 100mg/ml, with intra and interassay CV being 6.97% and 6.22% respectively.
出处
《放射免疫学杂志》
CAS
2000年第3期129-132,共4页
Journal of Radioimmanology