人瘦素放射免疫分析

Development of a Method of Radioimmunoassay for Human Leptin

应用重组人瘦素（Ｌｅｐｔｉｎ，ＬＰＴ），自行制备兔抗人ＬＰＴ抗血清，采用Ｉｏｄｏｇｅｎ法放射性核素标记物１２５Ｉ标记ＬＰＴ，建立了人血清ＬＰＴ的放射免疫分析（Ｒａｄｉｏｉｍｍｕｎｏａｓｓａｙ，ＲＩＡ）方法。研究结果表明，该法的特异性强，灵敏度高，重复好，该法与ＬＩＮＣＯ ＲＩＡ ｋｉｔ比较有显著相关性。检测范围：０．５～１００ｎｇ／ｍｌ。批内误差ＣＶ６．９７％，批间误差ＣＶ６．２２％。

Human leptin antihserum was prepared in our laboratory by immunizing rabbits with purified re- combinant human leptin while 125I - leptin was obrained by iodogen method. A method of RIA for human leptin was developed, using these self - made preparations. The result of tests showed high sensitivity and specificity with reliable reproducibility, which proved that the quality of our preparations was no less good than that of the imported LINCO kit. The detectabble range was between 0.5 - 100mg/ml, with intra and interassay CV being 6.97% and 6.22% respectively.