全血室温放置后有效成分变化的研究

Preliminary Study on Whole Blood Preserving at Room Temperature

目的探讨提高保存温度和延长保存时间后，对全血有效成分质量的影响。方法选择2012年5月本中心从体检合格的10例健康献血者采集的全血10袋200mL作为研究对象。将每袋全血平均分成2份，分别于22℃保存8h和24h，分别纳入对照组（n=10）和研究组（n=10）。两组全血均分离血浆冰冻保存，并分离红细胞（RBC）悬液于4℃保存，检测冰冻血浆凝血因子含量和保存期内RBC悬液相关质量指标变化。结果研究组分离的冰冻血浆较对照组凝血因子Ⅷ（FⅧ）含量显著降低（t=6．459，P〈0．05）；RBC悬液保存至第1和7天时，三磷酸腺苷（ATP）和pH值显著下降（P〈0．05），第14天起K＋浓度显著升高（P〈0．05），游离血红蛋白（FHb）有升高趋势，但差异无统计学意义（P〉0．05）。结论全血室温放置24h不宜制备新鲜冰冻血浆，对RBC质量影响在可接受范围内，应适当缩其短保存期。

Objective To research quality changes on plasma and red blood cell （RBC） separated from whole blood preserved at a higher temperature and a longer period. Methods In May 2012, a total of 10 bags of 200 mL whole blood which collected from 10 eases of healthy blood donors in our blood center were included in this study. All samples were separated into aliquot bags, then be divided parallel into two groups, the control group （n=10） was reserved at 22℃ for eight hours, the study group （n： 10） was reserved at 22℃ for twenty four hours. All the whole blood bags of each group were prepared into frozen plasma and packed RBC. Frozen plasma were stored frozen and packed RBC were preserved at 4℃ for 35 d. Coagulation factors of frozen plasma and adenosine triphosphate （ATP）, pH, free hemoglobin （FHb）, K＋ of packed RBC were tested. Results In study group, FⅧ of frozen plasma significantly decreased （t=6. 459,P〈0.05）. The ATP and pH value of packed RBC showed comparable lower on the first day and seventh day, the potassium concentration rose greatly on the fourteenth day, the FHb value had an upward tendency but not a significant difference. Conclusions Whole blood preserving at 22℃ for twenty four hours was not appropriate for preparation of fresh frozen plasma, its adverse effects on packed RBC had reached acceptable levels, a shorter preserving period of packed RBC on this condition was suggested.