透明质酸钠对硬膜外疤痕中胶原代谢影响的实验研究

An Experimental Study of the Influence of Sodium Hyaluronate on Collagen Metabolism of Epidural Scar

目的 :探讨透明质酸钠对硬膜外疤痕中胶原成分影响。方法 :采用兔非相邻节段椎板切除模型 ,分透明质酸钠组和生理盐水组 ,2、4、8周取材作透射电镜、ELISA和免疫组化分析。结果 :透明质酸钠组成纤维细胞分泌功能较差 ,胶原纤维疏松 ,且Ⅰ /Ⅲ胶原含量比低于对照组。 4、8周透明质酸钠组Ⅰ型胶原染色较浅 ,各时期透明质酸钠组Ⅲ型胶原染色均较深。结论 :透明质酸钠能提高伤口中疤痕Ⅲ型胶原含量 ,提示其能减少硬膜外疤痕形成 。

Objective: To study the effects of sodium hyaluronate on collagen component of epidural scar.Method:In this study,two level noncontiguous laminectomy of rabbit was performed and the rabbits were divided into sodium hyaluronate group and saline group.Animal were killed at 2,4,8 weeks after operation.The specimens were analyzed with transmission electron microscope,ELISA and immunohistochemical methods.Results:In sodium hyaluronate group,the fibroblasts was inactive and the collagenas loose.The ratios of type Ⅰ and type Ⅲ collagen in the sodium hyaluronate group was significantly lower than the control group.The intensity of collagen Ⅰ immunohistochemical stain in sodium hyaluronate group was lighter than the control group at 4 and 8 weeks,but the intensity of collagen Ⅲ immunohistochemical stain was darker than the control group.Conclusions:Sodium hyaluronate can increase the type Ⅲ collagen content of scar,imply that sodium hyaluronate has a beneficial role in wound healing and can reduce epidural scar formation.