β-七叶皂甙钠对肢体缺血再灌注损伤的保护作用

Protective Effect of β-Aescin on Ischemia and Reperfusion Injury of the Limbs

目的 :观察 β 七叶皂甙钠对肢体缺血再灌注损伤的保护作用。方法 :用兔造成肢体缺血再灌注损伤动物模型。实验分对照组、缺血再灌注组和 β 七叶皂甙钠组。取血浆测定丙二醛、肌酸磷酸激酶、谷草转氨酶和乳酸脱氢酶含量。取骨骼肌标本测定丙二醛、髓过氧化酶活性、肌细胞线粒体钙含量和组织湿 /干重比值。结果 :缺血再灌注组与对照组比较 ,血浆和骨骼肌的各项生化指标显著增高 (P <0 .0 1) ;使用 β 七叶皂甙钠后 ,血浆及骨骼肌各项测定指标较缺血再灌注组相比明显降低 (P <0 .0 5 ,P <0 .0 1)。结论 :β 七叶皂甙钠可减轻肢体缺血再灌注损伤 。

Objective: To observe the protective effect of β Aescin on limb ischemia and reperfusion injury.Methods:The experimental model of ischemia and reperfusion injury of limbs was produced in the rabbits which were divided into the control group,the ischemia and reperfusion group and the β Aescin group.The levels of MDA、CPK、GOT and LDH in plasma and MDA、MPO、mitochondria calcium and wet/dry of skeletal muscle were measured.Result:The biochemical indexes of plasma and skeletal muscle were dramatically higher than those in the control group(P<0.01),while the indexes of the β Aescin group were significantly lower as compared with those of ischemia and reperfusion group(P<0.01,P<0.05 respectively).Conclusion:The experiment revealed that β Aescin have protective effect on the muscle cells at the time of ischemia and reperfusion injury of the limb.