供者单采外周血中幼稚粒细胞检测的研究

A STUDY OF MEASUREMENT OF DONOR'S IMMATURE GRANULOCYTE COLLECTED PERIPHERAL BLOOD MONONUCLEAR CELL

目的 :探讨异基因外周血干细胞移植 ( Allo-PBSCT)供者经细胞集落刺激因子动员后 ,采集的外周血干细胞 ( PBSC)移植物中的幼稚粒细胞与单个核细胞 ( MNC)及 CD+ 3 4 细胞数之间相关性和移植的剂量标准。方法 :对 6例 Allo-PBSCT供者用 G-CSF或 G-CSF+GM-CSF进行动员 ,于动员前及动员后 ,对外周血及收集的MNC收集物中幼稚粒细胞及 CD+ 3 4 细胞进行检测计数 ,并计算出输给患者每公斤体重的 MNC、幼稚粒细胞及 CD+ 3 4细胞数。结果 :动员后外周血中的幼稚粒细胞与 CD+ 3 4 细胞数同步增加 ,外周血 MNC中幼稚粒细胞数与 CD+ 3 4 细胞数之间有较好的相关性。 6例患者全部植活恢复造血功能 ,并为染色体核型或血型转为供者型所证实。结论 :Allo-PBSCT供者经 G-CSF或 G-CSF+GM-CSF动员之后 ,外周血 MNC中幼稚粒细胞与 CD+ 3 4 细胞数有较好的相关性。因此 ,MNC与幼稚粒细胞数结合可作为 Allo-PBSCT的剂量标准 ,该法检测方便快速 ,稳定性好 。

Objective:To study the correlation among the immature granulocyte,mononuclear cells (MNC) and CD + 34 cell count in collected peripheral blood stem cell (PBSC) as well as the dosage standard of allogeneic PBSC transplantation (Allo PBSCT) after the donors were mobilized by G CSF or G CSF+GM CSF. Methods:Before and after six donors of Allo PBSCT mobilized by G CSF or GM CSF,measurements of the count of immature granulocyte and CD + 34 cell in collected MNC and peripheral blood was performed.We calculated the count of MNC,immature granulocyte,and CD + 34 cell by per kilogram of weight.Results:After mobilization,the immature granulocyte and CD + 34 cell count increased simultaneously in peripheral blood.The immature granulocyte count in peripheral blood MNC was well correlated to the CD + 34 cell.All patients were engrfted and got hematopoietic reconstitution,and complete donor engraftment documented by which the sex chomosome keryotype or blood type were changed to that of the donors.Conclusion:After the donors were mobilized by G CSF or G CSF+GM CSF,the immature granulocyte count of MNC in peripheral blood was well correlated to the CD + 34 cell,so,the combination of immature granulocyte and MNC can be the dosage standard of Allo PBSCT.This method was quick,stable,and conveninent to be applied widely.