江苏中部地区鹅副粘病毒的分离与鉴定

Isolation and Identification of Goose-derived Paramyxovirus in Central Area of Jiangsu Province

[目的]调查江苏省泰州市某鹅场鹅大批死亡的原因。[方法]通过流行病学调查、临床诊断、病理变化、动物回归感染试验、血清学检查等对江苏省泰州市某鹅场发生的疑似鹅副黏病毒病的病料进行病原分离与鉴定。[结果]该病毒能使SPF鸡胚以及10日龄鹅和鸡全部死亡,能凝集鸡红细胞并能被NDV标准阳性血清所抑制,不能被禽流感病毒标准阳性血清所抑制。发病死亡与人工感染死亡的临诊症状和病理变化基本一致。经电镜观察发现该病毒颗粒呈圆形,直径在150 nm左右,有囊膜及纤突。该分离毒株经灭活后免疫鹅群,取到良好的防治效果。[结论]该病毒为禽Ⅰ型副黏病毒,属副黏病毒科副黏病毒属。

[ Objective ] The research aimed to find out the death causes of a large number of geese in a goose farm in central areas of Jiangsu Province. [ Method] The virus of the suspected disease materials by goose paramyxovirus disease in a goose farm in Taizhou City of Jiangsu Province was isolated and identified by epidemiological investigation, clinical symptom, pathological changes, animal regression infection test and serological examination. [ Resuh] This virus was lethal to SPF chicken embryos, lO-day-old goose and chicken, which resulted in 100% mortality. This vurus was able to agglutinate chicken red blood cells, but it was inhibited by newcastle disease virus antiserum. But it could not be inhibited by avian influenza virus antiserum. Pathomorphologic changes of paramyxovirus infection in goose were observed to be basically i- dentical between naturally infected goose and artificially infected goose. The electron microscopic observation results showed that the virus granule was round and its diameter was about 120 nm ,with envelope and spike. The isolated virus was inactivated and immunized geese, and good control effects were＇achieved. [ Conclusion] This virus was avian I -tvoe oaramyxovirus, belonging to Paramyxovirus of Paramvxoviridae.