欧美杨108离体叶片和茎段再生体系的优化

Optimization of Regeneration System of Leaf and Stem for Populus euramericana 108

[目的]优化欧美杨108的无性繁殖再生体系的培养条件。[方法]以欧美杨108无菌苗为材料,采用正交试验优化叶片直接分化和茎段愈伤组织分化再生体系的培养条件。[结果]欧美杨108的叶片适宜在光照下进行直接再生分化,茎段适宜在光照下进行愈伤组织诱导再生分化。叶片不定芽分化的培养基为MS基本培养基(琼脂7.00 g/L、pH 6.00、蔗糖20 g/L)附加0.60 mg/L 6-BA和0.20 mg/L NAA;茎段愈伤组织诱导培养基为WPM固体培养基附加0.75 mg/L KT和1.50 mg/L 2,4-D。不定芽诱导生根培养基为WPM固体培养(蔗糖30 g/L)附加2.00 mg/L IBA。[结论]优化了欧美杨108叶片的直接分化再生体系和茎段愈伤组织再生体系的培养条件,为其组织培养及遗传转化体系的构建提供了基础支持。

[ Objective ] The aim was to optimize the culture conditions of asexual reproduction system for Populus euramericana 108. [ Meth- od ] Based on the orthogonal test design, the leaf and stem of aseptic seeding were taken as explants to study the optimization of regeneration system with direct differentiation from leaves and from callus differentiation from stems for Populus euramericana 108. [ Result] Leaves of Pop- ulus euramericana 108 direct regenerate and differentiate appropriately under illumination, while stem segments prefer to regenerate and differ- entiate after callus induction under illumination. The differentiation medium of adventitious buds from leaves is MS medium （ agar 7.00 g/L, pH 6.00, sucrose 20 g/L） added with 0.60 mg/L 6-BA and 0.20 mg/L NAA ; Callus induction medium of stem segments is WPM solid medi- um added with 0.75 mg/L KT and 1.50 mg/L 2, 4-D. Induction medium for rooting of adventitious buds is WPM solid culture （ sucrose 30 g/L） added with 2.00 mg/L IBA. [ Conclusion] The culture conditions for regeneration system of differentiation direct from leaves and from callus of stems were optimized, which provides a basis support for its construction of tissue culture and genetic transformation system.