1株高效油脂降解菌的筛选鉴定及其特性研究

Screening and Identification on Oil-Degradation Bacteria and Study of Characteristics

[目的]从自然环境中筛选鉴定高效降解油脂菌,并对其特性进行研究。[方法]从一学校食堂下水道口土壤中筛选分离得到1株高效降解油脂的菌株,经16SrDNA同源性序列分析鉴定,并对其降解条件进行研究,[结果]试验鉴定出该筛选菌株为地衣芽孢杆菌,以大豆油作为碳源,温度为35℃,最初pH为8,摇床速率为150 r/min,培养48 h后时对油脂的降解率87.4%。[结论]筛选得到的菌株用于含油废水的生物处理。

[ Objective ] To screen and identify high-effective oil-degradation bacteria from nature environment, and study on its characteris-tics. [ Method] One bacterial strain with high-oil degradation ability was screened from a school restaurant sewer soil. It was identified by 16SrDNA sequence homology analysis, and its degradation conditions were studied. [ Result] The strain was identified as Bacillus lichenifor- m/s, with soybean oil as carbon source, temperature 35℃, initial pH 8, agitation 150 r/min, the degradation on bean oil reached 87.4% af- ter culturing 48 h. [ Conclusion] The screened bacteria are prober for biological treatment of oily wastewater.