江苏省居民电压依赖钾通道蛋白1基因多态性与2型糖尿病的相关性

Association of polymorphisms of potassium voltage-gated channel, KQT-like subfamily, member 1 and type 2 diabetes in Jiangsu province, China

目的探讨江苏省居民电压依赖钾通道蛋白1（KCNQ1）基因多态性与2型糖尿病发病的关系。方法研究对象分别来自于江苏省无锡市2007年2型糖尿病队列研究和南通市2009年慢性非传染性疾病横断面调查，共有病例2925例和对照3281名，对其进行流行病学问卷调查及体格检查，并于清晨采集空腹10h静脉血5ml，应用TaqManOpenArray基因分型系统和i-PLEX Sequenom MassARRAY基因分型平台进行KCNQ1基因分型。分析调整年龄、性别和BMI后KCNQ1基因4个位点多态性与2型糖尿病发病风险的关系。结果调整年龄、性别和BMI后，KCNQ1基因rs2237897、rs2237892、rs2237895位点C等位基因的存在均增加了2型糖尿病的患病风险，相加模型的OR（95％CI）值分别为1．41（1．30—1．54）、1．35（1．24～1．47）和1．22（1．12～1．33）（P值均〈0．05）。按年龄、性别、BMI进行分层后，各层间rs2237897位点C等位基因增加了2型糖尿病的患病风险，≤56和〉56岁相加模型的OR（95％CI）值分别为1．39（1．22～1．59）、1．43（1．28～1．60）；女性和男性的OR（95％cI）值分别为1．40（1．26～1．55）、1．44（1．26～1．66）；BMI〈24kg／m2和≥24kg／m2的OR（95％CI）值分别为1．48（1．33～1．66）、1．34（1．17—1．53）（P值均〈0．05）。结论KCNQ1基因rs2237897、rs2237892、rs2237895位点多态性与江苏省汉族居民2型糖尿病的发生相关。

Objective To study the association of polymorphisms in the potassium voltage-gated channel,KQT-like subfamily, member 1 （KCNQ1） gene with type 2 diabetes in Chinese population from Jiangsu province. Methods Subjects consisting of 2925 cases and 3281 controls were enrolled from a community based cohort study of type 2 diabetes in Wuxi in 2007 and a community based cross-sectional survey on chronic non-communicable disease in Nantong in 2009. Epidemiological questionnaire survey and physical examinations were conducted and 10 h overnight fasting blood samples of 5 ml were drawn for all subjects. Genotypes were determined by TaqMan OpenArray Genotyping System and i-PLEX Sequenom MassARRAY platform. The relationship between KCNQ1 gene polymorphism and risk of type 2 diabetes after adjustment for age, sex and body mass index （BMI） was analyzed. Results The C allele of rs2237897, rs2237892 and rs2237895 at KCNQ1 increased the risk of type 2 diabetes with adjusted OR （95% CI） value being 1.41 （ 1.30 - 1.54）, 1.35 （ 1.24 - 1.47 ）, 1.22 （ 1.12 - 1.33 ） respectively （ all P value 〈 0. 05 ） under the additive genetic model after adjusted by age, sex and BMI. Stratification analyses in additive genetic model showed that the C allele of rs2237897 increased the risk of type 2 diabetes in subgroups stratified by age （ ≤56 years and 〉56 years） ,sex （females and males） ,BMI （ 〈 24 kg/m2 and I〉 24 kg/m2） with OR （95% CI） value being 1.39 （1.22 - 1.59）, 1.43 （1.28 - 1.60）, 1.40 （1.26 - 1.55）, 1.44 （1.26-1.66）,1.48 （1.33 - 1.66）, 1.34 （1.17 - 1.53） respectively （all P value 〈 0.05）.Conclusion Polymorphisms of rs2237897 ,rs2237892 and rs2237895 in the KCNQ1 gene were associated with occurrence of type 2 diabetes among Jiangsu province population.