骨髓基质细胞移植对大鼠脊髓损伤后COX-2基因表达的影响

Effect of bone marrow stromal cells transplantation on expressions of COX-2 gene in rats following spinal cord injury

目的观察骨髓基质细胞(bone marrow stromal cells,BMSCs)移植对大鼠脊髓损伤后COX-2 mRNA表达的影响。方法分离培养BMSCs并传代。取Wistar雌性大鼠72只随机分为3组:A组18只(假手术组)、B组27只(细胞培养基对照组)和C组27只(BMSCs组)。B组和C组脊髓损伤后进行DMEM或BMSCs损伤周围区注射。应用RT-PCR法和免疫荧光方法检测损伤脊髓组织内COX-2 mRNA的表达及COX-2蛋白分布情况。结果 COX-2 mRNA在A组大鼠脊髓组织中低水平表达。脊髓损伤移植术后,C组COX-2 mRNA表达水平均显著高于相应时间点的B组(P<0.01)。免疫荧光结果显示,A组COX-2仅表达于脊髓内的血管。脊髓损伤后,B组和C组COX-2表达分布则发生变化,主要见于损伤周围区脊髓内的血管和少数脊髓腹侧灰质内的神经元。结论 BMSCs移植能够增加脊髓损伤后COX-2 mRNA的表达。

Objective To observe the effect of bone marrow stromal cells（BMSCs） transplantation on expression of COX-2 mRNA in rats following spinal cord injury.Methods BMSCs were isolated and secondarily cultured.Male Wistar rats were randomly divided into sham-operated group（group A,n=18）,DMEM group（group B,n=27）,and BMSCs group（group C,n=27）.Rats in groups B and C were injected with BMSCs and DMEM after spinal cord injury.Expression of COX-2 mRNA and distribution of COX-2 protein in rat spinal cord tissue were detected by RT-PCR with immunofluorescence staining.Results The expression level of COX-2 mRNA was lower in group A than in groups B and C,and significantly higher in group C than in group B after BMSCs transplantation（P〈 0.01）.Immunofluorescence staining showed that the COX-2 was expressed only in spinal cord blood vessels of group A and mainly expressed in spinal cord blood vessels and in a small number of neurons in ventral gray matter of groups B and C.Conclusion BMSCstransplantation can up-regulate the expression of COX-2 mRNA in rats after spinal cord injury.