用于纳升级微量样品检测的原位激光诱导荧光系统

In-situ laser-induced fluorescence system for nanoliter sample detection

针对生命科学研究中测试样品量极少的关键问题,以毛细管作为样品检测池,采用入射光与荧光原位进出的系统设计方案;以半导体泵浦固体激光器作为激发光源、高分辨紧凑型光谱仪作为探测器,构建了用于纳升级微量样品检测的原位激光诱导荧光系统。利用水溶液中罗丹明B浓度的测量来验证系统性能。结果表明,罗丹明B浓度在93pg/mL～15ng/mL范围内与荧光强度有良好的线性关系,相关系数为0.9982,检出限为28pg/mL,证实该系统具有低噪声、低试剂消耗、低检出限、高灵敏度和精确度等优点,可满足生物测试的实际使用要求。

Aiming at the key problem of detecting extra small volume sample in bioseience research, an in-situ laser-induced fluorescence system was constructed for nanoliter sample detec- tion. In this research, we used a capillary as detection cell, a diode-pumped solid state laser as light source, and a high resolution spectrometer as detector for constructing an in-situ laser-induced fluorescence system. In addition, the fluorescent photons emitted from a sample in the capillary were directly collected in the common end of bifurcated fiber where the excitation lights came from. An experimental design for rhodamine 13 detection has been performed for system validation. The results show that the concentrations have a good linearity with the fluorescence in- tensity in the range of 93pg/mL-15ng/mL. The correlation coefficient is 0. 9982 with a detection limit down to 28pg/mL. The newly built detection system satisfies the requests of biomass detection practice, and possesses advantages of low noise, low reagent consumption, high sensitivity and good precision.