TLR-4在炎症性肠病中的表达及临床意义

Expression of TLR-4 in patients with inflammatory bowel disease

目的:探讨TLR-4在炎症性肠病(IBD)发病中的作用及临床意义。方法:收集46例炎症性肠病患者肠黏膜标本,通过RT-PCR检测肠黏膜中TLR-4mRNA的表达量,ELISA检测NF-κB转录活性及ELISA检测血清中IL-8和IL-10的含量。并收集30例健康志愿者作为对照组。结果:UC及CD患者TLR-4mRNA的表达量明显高于对照组(2.851±0.43及1.87±0.31 vs 0.24±0.1,P<0.05);IBD患者肠黏膜中NF-κB转录活性明显增强,与对照组比较具有明显差异(P<0.05);IBD各组血清中IL-8的含量均高于对照组,UC组中IL-10含量明显低于对照组(P<0.05);同时,UC组患者TLR-4表达水平和IL-8呈正相关,和IL-10呈负相关。结论:TLR-4在IBD中高表达,其可能通过诱导NF-κB活化,增加IL-8的转录和表达和抑制IL-10的表达参与了IBD的发病。

Objective. To investigate the role of TLR-4 in the pathogenesis of inflammatory bowel disease. Methods. Mucous samples of 46 patients with inflammatory bowel disease and 30 healthy volun- teers were collected and analyzed. The level of TLR-4 was detected by RT-PCR, and activation of tran- scription of NF-κB was detected by ELISA. The serum levels of IL-8 and IL-10 was also detected by ELISA. Thirty healthy volunteers were also enrolled as control group. Result. TLR-4 gene expression was significantly increased in UC and CD patients compared with that of the normal controls（2. 851±0.43 and 1.87±0.31 vs 0. 24±0.1, P〈0.05）. As compared with control group,activation of transcription of NF- κB in patients with IBD was significantly increased （P〈0.05）. Serum levels of IL-8 in IBD patients weresignificant higher than that of the control group,while IL-10 level was lower. TLR-4 gene expression were positively correlated with IL-8,negatively correlated with IL-10. Conclusions： TLR-4 can induce activation of transcription factor NF- κ B, enhance IL-8 expression while reduce IL-10 expression , and is involved with the pathogenesis of inflammatory bowel disease.