卡巴胆碱对烧伤大鼠延迟复苏时补液量及脏器血流量的影响

Effect of Choline on the Kaba Organ Blood Flow Volume of Fluid Infusion and Delayed Resuscitation Burned Rats

目的研究卡巴胆碱对烫伤休克大鼠延迟复苏时补液量及脏器血流量的影响。方法将32只Wistar大鼠随机分为假烧伤组(A组)、标准组(B组)、对照组(C组)和卡巴胆碱组(D组),B、C、D组大鼠制备30%体表面积Ⅲ°烫伤烧伤模型。C组伤后半小时经口给予卡巴胆碱。烫伤后大鼠首先暴露颈静脉、动脉并切开插入无菌硅胶管,以微蠕动泵控制输入乳酸钠林格氏液,A组和D组按B组剂量或速度的1/2给予。A组只剪毛、行颈静脉、动脉切开插管,但不烫伤及补液。记录补液开始后0、1、2、4h各组的平均动脉压(MAP)、心率(HR)、血细胞比容(Hct);测定肝、肾、肠组织血流量。结果 D组2h、4h后可明显降低HR,提高MAP,改善烧伤休克大鼠早期各脏器的血流量。结论卡巴胆碱能改善烧伤休克大鼠早期脏器的血流量,减少补液量需求,有助于烧伤后休克及并发症的防治。

Objective To study the Kaba choline on burn shock in rats after delayed fluid resuscitation fluid vdume and organ blood flow in rats. Methods Wistar rats and false burn group （group A）, standard group （group B）, control group （group C）, Kaba choline group （group D）, B. C. D group rats preparation of 30% BSA third degree burn model. C group at half an hour after oral administration of choline Kaba. The scalded rats first exposed jugular vein, artery incision and inserted in- to a sterile silicone tube, with a micro peristaltic pump control input of lactated Ringer＇s solution, A group and D group according to the standard dose of choline or velocity of the given 1/2. A group only shearing, jugular vein, artery intubation, but not scald and rehydration. Recording fluid infusion was started after 0, 1, 2, 4h in two groups in mean arterial pressure （ MAP）, heart rate （HR）, hematoorit （Het）. Determination of hepatic, renal, intestinal tissue blood flow. Results In group D, 2h, 4H could significantly decrease HR, increase MAP, improve blood flow in rats with burn shock early every organ. Conclusion Kaba cholinergic improvement in burn shook in rats early organ blood flow, reduction of fluid volume demand, contribute to lhe prevention and treatment of complications after burn shock.