急性心力衰竭大鼠B型钠尿肽水平与肝功能的关系

Relationship between B-type natriuretic peptide level and liver function of rats with acute heart failure

目的:探讨急性失代偿期全心衰竭大鼠B型钠尿肽(BNP)与肝功能的关系,阐明肝功能的变化对评价心力衰竭患者治疗效果和预后的价值。方法:选择雄性健康SD大鼠60只,采用腹腔连续注射异丙肾上腺素方法建立大鼠急性失代偿期全心衰竭模型,成功造模后存活的大鼠中随机选取20只作为心力衰竭组,另选取20只正常大鼠为对照组。2组大鼠均行心彩检查,测量左室舒张末内径(LVEDD)和收缩末内径(LVESD),计算左室射血分数(LVEF);检测2组大鼠血清BNP水平、血清天冬氨酸氨基转移酶(AST)和γ-谷氨酰转肽酶(γ-GT)水平。结果:与对照组比较,心力衰竭组大鼠LVEF值显著降低(P<0.01),BNP值明显增高(P<0.01);血清AST和γ-GT明显升高(P<0.01)。分别以LVEF和BNP为自变量,以AST及γ-GT为因变量进行线性回归分析,γ-GT和AST水平随LVEF的下降而升高,呈负相关关系(λ=-0.765,λ=-0.789,P<0.01);AST和γ-GT随BNP水平升高而升高,呈正相关关系(λ=0.793,λ=0.648,P<0.01)。结论:急性失代偿期全心衰竭大鼠的心功能与肝功能均异常,LVEF水平下降,BNP、AST和γ-GT水平升高;BNP与AST、γ-GT呈正相关关系,LVEF与AST、γ-GT呈负相关关系,提示肝功能变化可作为评估心力衰竭患者治疗效果和预后的指标。

Objective To study the relationship between the level of B-type natriuretic peptide（BNP） and liver function of rats with acute decompensated heart failure and to explore the values of the changes of liver function in evaluation of curative effect and prognosis of the patients with heart failure.Methods 60 male SD rats were chosen and intraperitoneally injected with isoproterenol for establishment of acute decompensated heart failure models.20 rats were randomly selected from the survival rats after successful modeling as heart failure group.And 20 normal rats were selected as control group.All rats were underwent heart echocardiography check.The left ventricular end-diastolie inner diameter（LVEDD）,the left ventricular end-systolic diameter（LVESD）,and the left ventricular ejection fraction（LVEF） were caculated.The levels of serum BNP,aspartate aminotransferase（AST）,γ-glutamyl（γ-GT） were detected.Results Compared with control group,the LVEF level of the rats in heart failure group was decreased（P0.01）,the BNP level was increased（P0.01）,and the AST and γ-GT levels were significantly increased（P0.01）.When LVEF and BNP were regarded as independent variables,and AST and γ-GT were regarded as the dependent variables,the linear regression analysis was performed;the γ-GT and AST levels were elevated with the decreasing of LVEF level,they had a negative correlation（λ =-0.765,λ=-0.789）（P0.01）;the AST and γ-GT levels were elevated with the increasing of BNP level,and they had a positive correlation（λ=0.793,λ= 0.648）（P0.01）.Conclusion The heart function and liver function of the rats with acute decompensated heart failure are abnormale;the LVEF level is declined,and the BNP,AST and γ-GT levels are increased;the γ-GT and AST levels have negative correlation with LVEF,and the AST and γ-GT levels have positive correlation with BNP.It indicates that the changes of liver function can be used to evaluate the curative effect and prognosis of the patients with heart failure.