秦艽总环烯醚萜苷的抗炎作用及其机制

Anti-inflammatory mechanisms of Gentiana macrophylla iridoid glucoside

目的观察不同浓度秦艽总环烯醚萜(GMI)对各型各期炎症模型的影响,探讨其抗炎作用的可能机制。方法采用二甲苯致耳廓肿胀、醋酸诱发小鼠腹腔毛细血管通透性增高,蛋清致大鼠背部气囊滑膜炎,棉球诱发小鼠肉芽肿模型,探讨GMI的抗炎作用。称体质量测定各组小鼠耳的炎症肿胀程度及肉芽增生水平,用化学方法检测各组小鼠腹腔洗涤液中的伊文思蓝含量、滑膜炎渗出液容积、渗出液中的白细胞(WBC)计数和肿瘤坏死因子(TNF-α)含量,渗出液和血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果与结论与阴性对照组相比,GMI能够抑制二甲苯致小鼠耳廓肿胀、醋酸致小鼠毛细血管通透性的增加,以及小鼠棉球肉芽肿的形成;其较阴性对照组还能明显减少渗出液容积,降低渗出液中WBC、MDA、TNF-α含量及血清中MDA水平,并能提高渗出液和血清中SOD的活性。结论GMI能够减轻各期炎症形成,机制可能与抑制炎性因子的渗出、消除自由基和抑制脂质过氧化有关。

Objective To observe effects of different concentrations of the Gentiana macrophylla iridoid glucoside（GMI） in different period inflammatory models, and discuss the possibility of anti-inflammation. Methods The anti-inflammatory models of GMI were investigated in mice by methods of the capillary permeability increase （AIPCPI）, the cotton-pellet induced-granuloma（CPIG） xylene-induced auricle swelling （XIAS）, intraperitoneal injection of acetic acid-induced peritoneal capilnd egg white-induced balloon back synovial inflammation. Levels of XIAS and CPIG in mouse were detected by weighing. Levels of mouse peritoneal washing fluid in the content of Evans blue, synovitis exudate volume, white blood cells（WBC）, tumor necrosis factor（TNF-α）, the exudate and serum levels of superoxide dismutase （SOD） and malondialdehyde were determined by chemical method respectively. Result Compared with negative control group, levels of XIAS, AIPCPI and CPIG decreased significantly in GMI group. Levels of WBC, MDA and TNF-α significantly decreased in GMI group than those of negative control group, but the activity of the exudate and serum SOD increased significantly in GMI group compared with that of negative control group. Conclusion The GMI showed protective effects on inflammation in animal models, which might be mediated through inhibition of the exudation of inflammatory factors, the lipid peroxidation and the elimination of free radicals.