胆甾醇琥珀酰基白芨多糖的制备及其理化性质研究

Preparation and physical chemistry properties research of cholesterol succinyl Bletilla striata polysaccharide

目的对白芨多糖疏水改性,并测其分子量和研究其理化性质。方法以白芨多糖为原料,合成胆甾醇琥珀酰基-白芨多糖;用红外光谱对结构进行表征;用高效凝胶渗透色谱法测分子量。结果合成了胆甾醇琥珀酰基白芨多糖;分子量为650 kDa;其理化性质:pH 6.89;黏度130 mPa·s;等电点5.07;溶解度0.9 g。结论合成方法可行,得率稳定,为进一步扩大试验提供了研究基础。

Objective To determination the molecular weight and studies on its properties after hydrophobic modification of Bletilla striata polysaccharide. Methods Bletilla striata polysaccharide were used as the starting materials, which reacted with cholesterol succinate to afford a derivate of cholesterol succinate polysaccharide, characterizate its structure by infrared spectroscopy, determinate its molecular weight by Sephadex chromatography（HPGPC）. Results Aeholesterol succinate polysaccharide was synthesized： molecular weight 650 kDa,pH 6.89,viscosity 130 mpa,isoelectric point 5.07, solubilit 0.9 g. Conclusion The method of synthesis was feasible and stable, which provided valuable research base for expand test.