不结球白菜感染菌核病后4个生理生化指标分析

Analysis of several physiological and biochemical index induced by Sclerotinia sclerotiorum among different non-heading Chinese cabbages

选用4个对菌核病具有不同抗性的不结球白菜品种003-R-6、Y3-R-4、109-S-9和003-S-7为试验材料,于四叶一心期接种核盘菌,采用苗期琼脂块叶片接种法测定各品种对菌核病的抗性,并分别测定接种前后叶片中4个生理生化指标。结果表明,苗期琼脂块叶片接种法与田间自然诱发鉴定结果相一致,可有效反映抗、感品种的差异。在核盘菌影响下,4个抗、感病品种中不结球白菜的丙二醛(MDA)含量、游离氨基酸(FAA)总量、几丁质酶活性和β-1,3-葡聚糖酶活性均有所变化。其中丙二醛(MDA)含量、游离氨基酸(FAA)总量表现为感病品种增加幅度大于抗病品种,而几丁质酶活性、β-1,3-葡聚糖酶活性均表现为抗病品种增加幅度大于感病品种,说明各指标与品种抗病性强弱存在一定的相关性。

Non-heading Chinese cabbage （Brassica campestris ssp. chinensis Makino） varieties （003-R-6, Ya-R-4, 109-S-9 and 003-S-7） with different resistance to Sclerotinia sclerotiorum were selected as materials. At seedling stage, leaf agar disk inoculation method was used to test resistance to Sclerotinia sclerotiorum. Leaves were used to analyze the physiological and biochemical changes such as the content of malondialdehyde （MDA）, free amino acid （FAA）, the activity of chitinase and β-1, 3-glucanase. The results showed that leaf agar disk in- oculation method was highly correlated with field induction, and it could well represent the resistance and suscep- tibility of the tested varieties. In addition, physiological and biochemical changes were observed after the inoculation. The increase of MDA, FAA in the susceptible variety 003-S-7 was higher than that of resistant variety 003-R-6. On the contrary, the increase of chitinase and β-1, 3-glucanase activity in the resistant varieties was higher than that of the susceptible ones. The results also indicated that change of the content or activity of the physiological and biochemical characters after inoculation is correlated with Sclerotinia sclerotiorum resistance to some extent.