利用响应面分析法优化葛渣异黄酮提取工艺

Optimization of Extraction Technique of Isoflavones from Pueraria Root Residue via Response Surface Methodology

利用响应面分析法(RSM)对生产葛粉后的葛渣中异黄酮的超声波辅助提取条件进行优化。在单因素试验基础上,选取料液比、提取温度和超声提取时间三因素为自变量,以葛渣异黄酮提取率为响应值,根据Box-Benhnken试验设计原理采用三因素三水平的响应面分析法建立回归模型,得到超声波辅助提取葛渣异黄酮的最佳条件为乙醇浓度70%,料液比1︰17(g︰mL),提取温度70℃,提取时间26 min,提取两次,异黄酮提取率可达96.3%,与模型预测理论值差异不显著(P>0.05)。

The conditions of ultrasonic assisted extraction of isoflavones from pueraria root residue were optimized based on response surface mothodology（RSM）.On the basis of single-factor test,the mathematical regression model was established on the dependent variable（extraction rate of isoflavones） and independent variables（material-to-liquid ratio,extraction temperature and extraction time） by Box-Benhnken design and response surface methodology with three factors at three levels.The optimum extraction conditions were as follows： ethanol concentration 70 %,material-to-liquid ratio 1 ︰ 17（g ︰ mL）,extraction temperature 70 ℃,extraction time 26 min,extracting for 2 times.Under these conditions,the extraction rate of isoflavones from pueraria root residue is up to 96.3 %,which has no significant difference with the prediction value（P0.05）.