复合因素对小鼠病毒性肝炎湿热证的影响

Effect of Complex Factors on Mice Model of Virus Hepatitis with Damp-heat Syndrome

目的建立小鼠病毒性肝炎的湿热证模型,为中药应用于湿热证治疗提供模型基础。方法将小鼠随机分为正常组、单纯湿热组、病毒组、模型组。采用反转录-聚合酶链反应(RT-PCR)检测小鼠肝脏病毒含量。对各组小鼠进行症状、肝功能、肝脏病理学改变等方面比较。结果病毒组、模型组小鼠的肝功能存在不同程度的损害。2组肝组织均有不同程度病理改变,以模型组病变更严重。用RT-PCR检测造模后血清病毒滴度,病毒组、模型组无明显差异,病毒含量2.9×104～5.5×104拷贝数/mL。结论初步构建小鼠病毒感染的湿热证模型,该模型的发病条件、病变程度及主要症状、体征均近似于中医湿热证特征,操作简单,重复性好,有较高的应用价值。

Objective To establish the damp-heat syndrome model of hepatic virus infected mice,and provide animal model for TCM therapy in treating damp-heat syndrome.Methods Mice were randomly divided into normal group,damp-heat group,virus infection group and model group.RT-PCR was used to detect the hepatic virus concerntration in liver of mice,and symptoms,hepatic function,hepatic pathological change among groups were compared.Results Hepatic damages were found in the virus infection group and model group,pathological changes also happened to the liver tissues in these two groups,which of the model group was more severe.There was no significant difference in serum virus detected by RT-PCR between virus infection group and model group.Virus concentration was 2.9×1045.5×104 copies/mL.Conclusion The damp-heat syndrome mice model of hepatic virus infection was successfully established.The model can closely resemble the TCM damp-heat syndrome,with simple operation,and can be repeated easily,which implies big practical potential.