3种中药单体成分对肺念珠菌的抗菌活性

Anti-fungal Activity of Three Kinds of Chinese Herbal Monomers Against Pulmonary Candida

目的观察3种中药单体成分对肺念珠菌体外的抗菌活性。方法采用试管药基法,将桂皮醛、丁香酚、藿香酮倍比稀释成2.5～0.019 5μL/mL,对致病性肺念珠菌(白色念珠菌、热带念珠菌、克柔氏念珠菌)进行最低抑菌浓度(MIC)和最低杀菌浓度(MFC)实验观察。结果桂皮醛对白色念珠菌、热带念珠菌MIC为0.019 5μL/mL,克柔氏念珠菌为0.039 1μL/mL;白色念珠菌、热带念珠菌MFC为0.039 1μL/mL,克柔氏念珠菌为0.078 11μL/mL。丁香酚对3种念珠菌MIC为0.078 1μL/mL,对白色念珠菌MFC为0.078 1μL/mL,热带念珠菌、克柔氏念珠菌为0.156 2μL/mL;藿香酮对白色念珠菌、热带念珠菌MIC为0.156 2μL/mL,克柔氏念珠菌为0.312 5μL/mL;对白色念珠菌MFC为0.156 2μL/mL,热带念珠菌、克柔氏念珠菌为0.312 5μL/mL。对照组氟康唑对白色念珠菌、热带念珠菌MIC为0.313μL/mL,对克柔氏念珠菌为2.5 mg/mL。结论 3种中药单体成分对念珠菌均有很强的抗菌作用,其抗菌作用由强到弱依次为桂皮醛、丁香酚、藿香酮。克柔氏念珠菌对氟康唑显示耐药。

Objective To explore the anti-fungal activity of three kinds of Chinese herbal monomers against pulmonary candida in vitro.Methods Based on the drug-based method,cinnamaldehyde,eugenol and pogotstone were proportionally diluted to 2.50-0.0195 μL/mL,and MIC and MFC on pulmonary candida including candida albicans（C.albicans）,candida tropicalis（C.tropicalis） and candida krusei（C.krusei） were determined.Results MIC of cinnamaldehyde against C.albicans and C.tropicalis were 0.0195 μL/mL,0.039 1 μL/mL for C.krusei,MFC was 0.039 1 μL/mL for C.albicans and C.tropicalis,0.078 1 μL/mL for C.krusei.MIC of eugenol against candida was 0.078 1 μL/mL,MFC was 0.078 1 μL/mL for C.albicans,0.156 2 μL/mL for C.tropicalis and C.krusei.MIC of pogotstone was 0.156 2 μL/mL for C.albicans and C.tropicalis,0.312 5 μL/mL for C.krusei,MFC was 0.156 2 μL/mL for C.albicans,0.312 5 μL/mL for C.tropicalis and C.krusei.MIC of fluconazole in the reference group was 0.313 μL/mL for C.albicans and C.tropicalis,and 2.5 mg/mL for C.krusei.Conclusion Three monomers of Chinese herbal have strong anti-fungal activity against candida（C.albicans,C.tropicalis and C.krusei）,and the effects are in the following descending order：cinnamaldehydeeugenolpogotstone.C.krusei is resistant to fluconazole.