摘要
目的寻找一种简单、高效的提取方法提取人血凝块中基因组DNA。方法分别用改良酚—氯仿提取方法和改良碘化钾提取方法提取人血凝块基因组DNA。结果 PCR结果显示,改良酚—氯仿提取方法较改良碘化钾提取方法提取的DNA总量明显较高,提取效率高,PCR扩增的效果好。结论改良酚—氯仿提取方法制备的DNA效果较好是从血凝块中提取基因组DNA中首选考虑的方法。
Objective To compare the effectiveness of two methods for extracting human genome DNA from human blood clots in terms of DNA quantity, quality and efficiency of PCR-based reaction. Methods The improved phenol-chloroform method and the improved potassium iodide method were used to extract human genomic DNA from whole blood. Resuits The PCR result showed that the improved phenol-chloroform method was more effective with increasing DNA quantity, high efficiency of extraction, effective PCR amplification. Conclusions The improved phenol-chloroform method is a better one for gaining large quantity of PCR amplification. This method deserves to be the first choice for extracting human genomic DNA from human blood clots.
出处
《山东医药》
CAS
2013年第19期33-35,共3页
Shandong Medical Journal
基金
河南省科技厅立项课题项目(072103810403)
