蟾酥水提取物对人宫颈癌HeLa细胞生物学活性的抑制作用

Inhibitory effect of Venenum Bufonis on the bidogical activity of human HeLa cells

目的:研究蟾酥水提取物对子宫颈癌HeLa细胞黏附、迁移和侵袭能力的抑制作用。方法:应用MTT法检测蟾酥水提取物对子宫颈癌HeLa细胞活力和细胞黏附能力的影响,划痕实验观察蟾酥水提取物对HeLa细胞运动能力的影响,Transwell小室模型研究蟾酥水提取物对HeLa细胞的迁移和侵袭的抑制作用,免疫印迹法检测基质金属蛋白酶-9(MMP-9)的表达。结果:100μmol/L或以上浓度的蟾酥水提取物作用24 h,Hella细胞生存率明显低于对照组(P均<0.05);20μmol/L或以上浓度的蟾酥水提取物作用24 h,HeLa细胞黏附率明显低于对照组(P均<0.05)。20μmol/L或以上浓度的蟾酥水提取物对Transwell小室膜上HeLa细胞的运动、迁移、侵袭均有抑制作用。50μmol/L蟾酥水提取物作用下,HeLa细胞的MMP-9蛋白表达强度比对照组明显减弱。结论:蟾酥水提取物能抑制HeLa细胞的黏附,阻止其迁移和侵袭,抑制MMP-9的活性可能是蟾酥水提取物体外诱导HeLa细胞发生凋亡的重要作用机制之一。

Objective ： To investigate inhibitory effects of ~enenum Bufonis on the adhesion, invasion and mi- gration of human cervical carcinoma HeLa cell line. Methods： The viability of HeLa cells was measured by MTF assay. Cell adhesion assay, cell scratch assay and Transwell chamber assay were used to determine changes in ad- hesion, invasion and migration of HeLa cells after Venenum Bufonis treatment. Western blot was used to detect the expression of matrix metalloproteinase-9 （MMP-9）. Results： The viabilities of HeLa cell treated 24h by 100 μmol/L or above Venenum Bufonis were lower than that of control group （all P 〈0. 05 ）. And the adhesion rates of HeLa cell treated by 20 μmol/L or above Venenum Bufonis was lower than that of control group （ all P 〈 0. 05 ）. 20 μmol/L or above Venenum Bufonis could obviously suppress the invasive and migrativeability of HeLa cells. Furthermore, MMP-9 protein expression of Hela treated by 50 μmol/L Venenum Bufonis was significantly de- creased than that of control group. Conclusion： It shows Venenum Bufonis can inhibit the adhesion, invasion and migration of HeLa cells, which may be associated with down-regu-lation of the expression of MMP-9.