摘要
采用PHA和秋水仙素活体注射方法制备了中华沙鳅头肾组织染色体标本并分析其核型。结果表明,中华沙鳅二倍体染体色数目为96,核型公式为:2n=8m+12sm+20st+56t,臂数NF=116,无异形染色体分化。通过与其他鳅科鱼类染色体核型比较,推测中华沙鳅核型是鳅科祖先经过罗伯逊易位、染色体类型转变、染色体多倍化等途径进化而来。
The metaphase chromosomes of Sinibotia superciliaris was obtained from head kidney tissue by the method of phyohemagglutinin （PHA） and colchicine injection, and then the karyotype was analyzed. The results showed that there were 96 chromosomes in the diploid and the karyotype formula was 2n=8m＋12sm＋20st＋56t, NF=116. No sexual chromosome or heteromorphic chromosome was observed. By comparison with the chromosome karyotypes of other Cobitidae species, karyotype of S. superciliaris might evolve from the type of Cobitinae ancestors by Robertsonian translocation, translation of chromosome types and chromosome polyploidy.
出处
《四川动物》
CSCD
北大核心
2013年第3期385-388,共4页
Sichuan Journal of Zoology
基金
四川省教育厅科技创新科研团队基金(KYTD201009)
内江市科学技术知识产权局科技支撑计划项目(11028)
四川省教育厅项目(12ZA246)资助
关键词
中华沙鳅
染色体
核型
Sinibotia superciliaris
chromosome
karyotype
