泽陆蛙消化道5-羟色胺细胞的免疫组化定位

Immunohistochemical Localization of 5-Hydroxytryptamine-producing Cells along the Digestive Tract of Fejervarya multistriata

应用SABC免疫组织化学法对泽陆蛙消化道5-羟色胺细胞进行了鉴别和定位研究,并统计分析了5-羟色胺细胞沿消化道各段的分布密度。结果表明,消化道5-羟色胺开放型和闭合型细胞的比值变化范围为0.71～1.50,食管和直肠以开放型细胞为主,十二指肠两种细胞的比值接近于1,其余部位以闭合型细胞为主。分布密度直肠和十二指肠最高,贲门和胃体最低。泽陆蛙消化道5-羟色胺细胞的形态学特点与其他两栖动物相似,但分布密度有自身的独特性,这可能与其食性和生活习性有关。

To identify and localize the 5-hydroxytryptamine （5-HT）-producing cells in the digestive tract of Fejervarya multistriata, strep avidin-biotin-peroxidase complex method was performed, and the distributive density of 5-HT cells was compared among different portions. The results showed that the ratio between open and closed populations of 5-HT cells was ranged from 0.71 to 1.50 in different portions of the digestive tract. Esophagus and rectum were mainly comprised by open populations of 5-HT cells and other portions were closed populations. The ratio between open and closed populations of 5-HT cells in the duodenum was almost equal to 1. Rectum and duodenum showed the highest distributive density of 5-HT cells and stomachus cardiacus and corpus the lowest. Although the morphological features of 5-HT cells in the digestive tract of F. multistriata were similar to those of other amphibians, the unique characteristic of distributive density might be related to the food habit and life style.