通入CO_2和补加氮源对紫球藻生长和代谢物质积累的影响

Effect of CO_2 concentration and nitrogen source addition on growth and major metabolic substances accumulation in Porphyridium sp.

论文研究了柱状光生物反应器中CO2浓度和氮源添加模式等培养条件对紫球藻(Porphyridium sp.UTEX 637)生长和主要代谢产物积累的影响。结果表明,通入CO2能显著促进紫球藻的生长及胞外多糖、水溶性蛋白和总脂等生物活性物质的积累,其中1%的CO2浓度对紫球藻的生长及活性物质积累最有利,该条件下紫球藻的最高干重可达到8.14 g/L,是对照组的4.93倍;胞外多糖产量明显高于对照组,最高可达到238.8 mg/L;补加氮源KNO3对紫球藻生长及胞外多糖、水溶性蛋白含量均有明显的促进作用,最高干重、胞外多糖含量可分别达到对照组的1.5倍和1.25倍,但不利于总脂的积累。

The effects of CO2 concentration and nitrogen source KNO3 addition on the growth and major metabolic substances accumulation of Porphyridium sp.UTEX 637 were researched by culturing it in the Φ6×60cm cylindrical glass photobioreactor.The results showed that appropriate concentration of CO2 could significantly promote the algal biomass and the content of exopolysaccharides,water-soluble protein and total lipids.The most suitable CO2 concentration was 1%.The maximum cell dry weight of experimental group was 8.14 g/L（1%CO2）,which was 4.93 times of the control group.The maximum output of exopolysaccharides was 238.38 mg/L,which was significantly higher than the control group.Adding nitrogen source KNO3 had apparent stimulative effect on the algal cell growth,exopolysaccharides,and water-soluble protein content.The maximum cell biomass and exopolysaccharides content were 1.5 and 1.25 times of the control group,but it did not contribute to the total lipids accumulation.