基质金属蛋白酶-9基因单核苷酸多态性与冠心病遗传易感性的关系研究

Study on the relationship between single nucleotide polymorphisms of MMP-9 gene and genetic predisposition of coronary disease

目的分析金属蛋白酶-9（MMP-9）-1562C／T基因多态性在冠心病患者及非冠心病患者中的分布情况，了解MMP-9基因单核苷酸多态性与冠心病遗传易感性的关系。方法采用PCR-RFLR法对调查对象血液样本进行基因分型．分析MMP-9-1562C／T基因型频率和等位基因频率在病例组和对照组之间的差异。结果病例组与对照组CC／CT＋TT的OR值为0．83，差异无统计学意义（OR95％C10．51-1．36，P〉0．05）；C/T等位基因OR值为0．83，差异无统计学意义（OR95％C10．44-1．55，P〉0．05）。结论MMP-9基因单核苷酸多态性与冠心病遗传易感性的关系尚不能肯定．还需要今后进一步的研究证实。

Objective To analyze the distribution of MMP-9 -1562C/T gene polymorphism in patients with and with- out coronary disease （CAD）, in order to understand the relationship between single nucleotide polymorphisms of MMP- 9 gene and genetic predisposition of CAD. Methods PCR-RFLR was used to analyze the genetic typing of blood sam- ple in investigation objects, then the difference in MMP-9 -1562C/T genotype frequency and allele frequency between the cases group and the control group was analyzed. Results The OR value of MMP-9 -1562 CC/CT＋TT was 0.83 in the cases group and the control group, the difference was not significant （OR 95%CI 0.51-1.36, P 〉 0.05）; while, the OR value of C/T allele was 0.83, the difference was not significant （OR 95% CI 0.44-1.55, P 〉 0.05）. Conclusion The association between single nucleotide polymorphisms of MMP-9 gene and genetic predisposition of CAD can not be affirmed, which needs further study to confirm.