p21、p53基因表达及其单核苷酸多态性与肝细胞肝癌的关系

Relationship between p21,p53 expression and single nucleotide polymorphism with hepatocellular carcinoma

目的探讨肝细胞肝癌患者p21和p53基因的表达及其单核苷酸的多态性,分析其与肝细胞肝癌的关系。方法选择50例肝细胞肝癌患者和50名健康志愿者,采用PCR技术检测两组外周血白细胞中的p21和p53基因的表达情况,采用PCR-RFLP方法检测p21 3’UTR位点以及p53 intron 6位点的基因多态性。结果肝癌组p21和p53 mRNA表达显著高于对照组(P<0.01)。饮酒能够增加p53 mRNA的表达。p21基因3’UTRC/T位点C/T+T/T型与60岁以上人群肝癌患病率升高有关。结论 p21基因以及p53基因的高表达可能与肝细胞肝癌有一定的相关性,饮酒能够使肝癌患者p53基因的表达升高。

Objective To discuss the relationship between p21 ,p53 expression and single nucleotide polymorphism with hepatocellular carcinoma. Methods Fifty cases with hepatocellular carcinoma and fifty healthy volunteers were selected, Expression of p21 ,p53 were detected by PCR. Single nucleotide polymorphism of p21 3 ＇UTR and p53 in tron 6 were detedted by PCR-RFLP. Results p21 ,p53 mRNA expression of hepatocellular carcinoma group were high er than control group （P 〈 0.01）. Drinking could improve p53 mRNA expression.Single nucleotide polymorphism of p21 3＇UTR C/T＋T/T type was correlation with morbidity increasing of over 60 years old crowd. Conclusion High p21, p53 expression may is correlation with hepatocellular carcinoma. Drinking can improve p53 mRNA expression.