响应面法优化水酶法提取林蛙卵蛋白

Optimization on aqueous enzymatic extraction conditions of forest frog egg protein by response surface method

研究了利用碱性蛋白酶提取林蛙卵蛋白的酶解工艺。以林蛙卵蛋白提取率为指标,通过单因素实验和响应面试验,得到影响试验的因素依次为加酶量>料液比>酶解温度>酶解时间>酶解pH值。最佳的酶解参数为:加酶量1.9%,温度58℃,酶解时间4h,料液比1∶5,pH值8.8,此时林蛙卵蛋白提取率为9.63%。

Hydrolysis process of aqueous enzymatic to extract forest frog egg protein is studied. With the extraction rate as the index, both the single factor experiment and response surface experiment are applied to analyze the influential factors. The contribution rate of factors is as follows： enzyme amount 〉 the ratio of solid to liquid 〉 hydrolysis temperature 〉 hydrolysis time 〉 pH. The optimum conditions are： enzyme amount 1. 9%, hydrolysis temperature 58 ℃, hydrolysis duration 4 h, the ratio of solid to liquid 1 ： 5, pH8.8, the total extraction rate of forest frog egg protein can reach 9.63%.