摘要
研究了利用碱性蛋白酶提取林蛙卵蛋白的酶解工艺。以林蛙卵蛋白提取率为指标,通过单因素实验和响应面试验,得到影响试验的因素依次为加酶量>料液比>酶解温度>酶解时间>酶解pH值。最佳的酶解参数为:加酶量1.9%,温度58℃,酶解时间4h,料液比1∶5,pH值8.8,此时林蛙卵蛋白提取率为9.63%。
Hydrolysis process of aqueous enzymatic to extract forest frog egg protein is studied. With the extraction rate as the index, both the single factor experiment and response surface experiment are applied to analyze the influential factors. The contribution rate of factors is as follows: enzyme amount 〉 the ratio of solid to liquid 〉 hydrolysis temperature 〉 hydrolysis time 〉 pH. The optimum conditions are: enzyme amount 1. 9%, hydrolysis temperature 58 ℃, hydrolysis duration 4 h, the ratio of solid to liquid 1 : 5, pH8.8, the total extraction rate of forest frog egg protein can reach 9.63%.
出处
《长春工业大学学报》
CAS
2013年第2期137-144,共8页
Journal of Changchun University of Technology
基金
吉林省科技发展计划基金资助项目(20110252)
关键词
碱性蛋白酶
林蛙卵蛋白
响应面
林蛙卵蛋白提取率
alcalase
forest frog egg protein l response surface
extraction rate of forest frog egg protein.