环丙烯类乙烯效应抑制剂二次处理对番茄乙烯生成及其基因表达的影响

Effect of Twice Treatment with Different Cyclopropene Ethylene Inhibitors on Postharvest Ethylene Biosynthesis and Gene Expression of Tomato Fruits

为研究不同支链长度的3种环丙烯类乙烯效应抑制剂二次处理对番茄采后乙烯生物合成及乙烯生物合成关键基因表达的影响,分别采用0.75μL/L 1-MCP(1-Methylcyclopropene,1-甲基环丙烯),2μL/L 1-PentCP(1-pentylcclopropene,1-戊基环丙烯)和0.5μL/L 1-OCP(1-octylcyclopropene,1-辛基环丙烯)处理番茄,以不处理为对照,并于贮藏第8天进行了二次处理,对处理后番茄果实常温货架期间的乙烯生物合成及其基因表达情况进行了研究。结果表明:3种乙烯效应抑制剂处理均能不同程度地抑制番茄果实采后贮藏过程中的呼吸强度和乙烯释放量,同时抑制了ACS2和ACO1基因的表达量,上述效果又随支链长度的延长而呈现减弱的趋势;3种处理均较好地保持了番茄果实贮藏期间的品质,二次处理对果实贮藏特性影响不大。

Effect of twice treatment with three cyclopropene ethylene inhibitors with different 1 substituted branch chain lengths on postharvest ethylene biosynthesis and gene expression of tomato fruits(Solanum lycopersicum L.) were investigated in this manuscript.The fruits were packed with plastic bags(0.02 mm) after being fumigated for 20h with 0.75 μL/L 1-MCP(1 Methylcyclopropene),2 μL/L 1-PentCP(1-pentylcyclopropene) or 0.5 μL/L 1-OCP(1 octylcyclopropene) and then stored at ambient temperature(20 ℃) for maturation evalution,and second time treated wound be in 8 days as the first.The results show that treatments with any one of these three inhibitors,compared with that of the untreated fruits,reduced ethylene production and respiration rate were decreased and,the express level of ACS2 and ACO1 gene were lower in the fruits.Furthermore,these effects tended to decrease as the 1-substituted branch chain length of the cyclopropenes increased.In addition,treatments with any inhibitors delayed physiological metabolism and maintained fruit quality.Twice treatments,however,did not exert obvious effects.