交感神经去除对大鼠胃APUD细胞的影响

Effect of Sympathectomy on the APUD Endocrine Cells of Rat Gastric

目的:研究交感神经去除对胃内分泌细胞的影响,探讨降低迷走神经切除治疗十二指肠溃疡术后复发率的可能方法。方法:将30只Wistar大鼠随机分成2组,1组为模拟手术的对照组,2组行腹腔交感神经节切除,术后饲养6周后取材。测基础胃酸量、血清胃泌素水平,光镜下计胃粘膜中APUD细胞数、G细胞数、D细胞数,电镜下观察APUD细胞超微结构。结果:去除大鼠胃的交感神经后,(1)基础胃酸分泌量及血清胃泌素水平无明显变化;(2)胃的APUD细胞总数减少,G细胞减少,2组间有显著差异,D细胞无明显变化;(3)APUD细胞超微结构未见明显变化。结论:交感神经切除后,胃G细胞的减少可能是迷走神经合并交感神经去除后胃酸分泌恢复不全的原因之一。迷走神经切除治疗十二指肠溃疡时同时切除交感神经是降低术后复发率的可能方法。

Objective：Vagotomy has been performed for treating duodenal ulcer ,but its recurrence rateis very high,The gastric acid secretion always recovered completely to the preoperative level. But afterperformed vagotomy combined with sympathectomy,the gastric acid secretion didnt recover completely. For reserch the probable reasons,we performed isolated sympathectomy on rat,the hanges of the basal gastricacid output and the serum gastrin and the amounts of APUD cells、G cells and D cells of rat gastric wereobserved. Methods： Thirty Wistar rats were divided into 2 groups in random,group 1 is control group, group 2 is operated group which were performed the extirpation of the coeliac and superior mesenteric ganglia. Six weeks after the operation,all animals were sampled,the basal gastric acid output and serumgastrin were measured,and the amounts of APUD cells、G cells and D cells were counted with a light microscope,the ultrastructure of APUD cells were observed with a transmission electron microscope. Results：（1） Sympathetic denervation of the stomach did not influence the basal gastric acid secretion and serum gastrin level.（2）The amounts of total APUD cells and G cells of rat gastric mucosa reduced significantly,and D cells had little changes（.3）The difference of ultrastructure of APUD cells between two groups had not been found. Conclusion：After sympathectomy,the reduction of the amount of total APUD cells and G cells of rat gastric mucosa had been observed,there was significantly statistical difference between two groups. This change may be one of the reasons of the uncompleted recovery of gastric acid output after vagotomy combined sympathectomy.