延经丸对去卵巢大鼠模型骨密度及血清中E_2、T的影响

Effects of the Yanjing pill on bone density and E_2 and T of serum in the rat model

目的:本研究旨在通过延经丸对去卵巢模型大鼠骨密度、E2、T的影响进一步探讨延经丸对去卵巢骨质疏松模型大鼠的疗效,为临床治疗绝经后骨质疏松症提供科学数据。方法:SPF级Wistar雌性大鼠60只,随机分为假手术组(n=10)、空白模型组(n=10)、延经丸大剂量组(n=10)、延经丸小剂量组(n=10)、西药对照组(n=10)、中药对照组(n=10)。假手术组仅开腹腔见到卵巢,其余各组通过切除两侧卵巢建立大鼠模型,利用骨密度仪检测股骨骨密度值,酶联免疫吸附法(ELISA)测定血清中E2、T含量以及算出比值。以上实验数据采用SPSS17.0统计软件进行单因素方差(ANOVA)分析。结果:延经丸具有明显的治疗去卵巢骨质疏松模型大鼠的功效,初步提示了其为治疗绝经后骨质疏松症的有效药物,为以后临床提供了科学依据。

Objective： This paper was focused on effects of the Yanjing pill on the OP rat model for the meaning of treating the OP following menopause. Methods： 60 rats were divided into the psedo-sugery group and blank, high dose of Yanjing pill, little dose of Yanjing pill, modem medicine control and TCM medicine control groups. The paedo-surgery was to cut abdomen only, rats＇ovaries in the other groups were cut. Density of bone and E2, T was recorded for analysis. Results： The Yanjing pill has obvious clinical efficacy treating OP in ovary-free rats.