径向流色谱分离纯化海藻多糖及其抗氧化活性比较分析

Separation and Purification of Seaweed Polysaccharides by Radial Flow Chromatography and Comparative Analysis of Antioxidant Activities in vitro

以裂片石莼、亨氏马尾藻和海萝3种海藻为原料,采用超声波辅助提取、分步醇沉得到7种组分粗多糖,利用径向流色谱分离纯化粗多糖,测定超氧阴离子、羟基自由基和ABTS 3种自由基体系下其抗氧化能力。结果表明:通过径向流处理,多糖回收率和蛋白脱除率分别可达97.79%和87.32%;7种海藻多糖组分均具有抗氧化活性,均随多糖浓度的增加而增加,马尾藻60%醇沉组分(SHP60)抗氧化活性最高,在其70μg.mL-1浓度时,对超氧阴离子自由基和羟基自由基清除率分别为74.34%和71.84%;同种海藻的中等分子量多糖组分的抗氧化活性较强。

Three kinds of, sea algae including Ulva fasciata, Sargassum henslouianum, and Gloiopeltis furcata were selected as experimental materials. Seven kinds of different crude polysaccharides components were obtained through ultrasonic-assisted extraction and stepwise ethanol precipitation. The crude polysaecharides were isolated and purified using radial flow chromatography. The purified polysaccharides were evaluated on their antioxidant activities including superoxide radical assay, hydroxyl assay and ABTS assay in vitro. The results indicated that the polysaccharide recovery rate and the deproteinization rate was 97.79% and 87.32% respectively. All of the seven different polysaccharide components showed some degree of radical scavenging activities, which increasing with concentration. The highest activity was found in SHP60, polysaccharides component of Sargassum henslouianum precipitated by 60% ethanol. Its scavenging ratio for superoxide radical was 74.34% and hydroxyl radical was 71.84% respectively at the concentration of 70μg. mL-1. The polysaccharides with medium molecular weight had better scavenging activities than those of high and low molecular weight.