摘要
Cs-EV12(GenBank登录号:GH618817)为一受假眼小绿叶蝉取食诱导的茶树香豆酰辅酶A:莽草酸/奎宁酸香豆酰转移酶的cDNA片段,应用RACE技术克隆了该基因的全长cDNA,命名为CsHCT(GenBank登录号:JQ619537)。CsHCT cDNA序列全长1 653 bp,包含一个编码447个氨基酸的完整开放阅读框。序列分析显示CsH-CT与毛果杨、烟草、拟南芥的HCT在氨基酸序列上一致性分别为54%、41%和40%,含有植物BAHD酰基转移酶家族活性中心的保守基序,以及与HCT正确折叠有关的DFGWG基序。HCT的表达受假眼小绿叶蝉取食、低温、紫外线和茉莉酮酸甲酯的诱导。
Cs-EV12 is the cDNA fragment of a HCT gene of tea （ Camellia sinensis L） up-regulated by mild infestation of green leafhopper （Empoasca vitis Gtithe）. The full-length cDNA of Cs-EV12 was cloned by RACE （rapid amplification of cDNA ends）. This cDNA was designated as CsHCT （GenBank accession number： JQ619537）, which is 1 653 bp in length, and contains an open reading frame of 447 amino acids. CsHCT protein shares most amino acid sequence in similarity with the HCTs from Populus trichocarpa, Nicotiana tabacum and Arabidopsis thaliana, and the similarities are 54%, 41% and 40%, respectively. CsHCT contains the conserved motif involved in the catalytic activity of BAHD acetyltransferase, and DFGWG motif required for proper folding of the HCT polypeptide. The expression of CsHCT can be induced by leafhopper feeding, low temperature, UV-B irradiation and methyl jasmonate.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2013年第6期19-22,37,共5页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30570137)
河南省基础与前沿技术研究计划项目(092300410244)
关键词
茶树
HCT
基因表达
Tea （Camellia sinensis L）
Hydroxycinnamoyl-CoA： shikimate/quinate hydroxycinnamoyltransferase（HCT）
Gene expression
