金毛鳞伞液体基质与培养条件优化研究

Study on Optimization of Liquid Medium and Culture Condition of Pholiota aurivella

以金毛鳞伞一级菌种为试材,在单因素试验基础上进行正交实验设计,对金毛鳞伞液体基质与培养条件进行了优化研究。结果表明:金毛鳞伞最适培养基组合为可溶性淀粉2.0%、蛋白胨0.5%、K2HPO40.3%、MgSO40.3%;金毛鳞伞的最适液体培养条件为温度26℃、摇床转速150r/min、pH为6.0、接种量15%。

Taking the first class inoculums of Pholiota aurivella as material,the optimization of liquid medium and culture condition of Pholiota aurivella were studied by orthogonal experiment based on the single factor test.The results showed that the most suitable culture medium combination were as follows：2.0% glucose,peptone 0.5%,K2HPO4 0.3%,MgSO4 0.3%;the most suitable liquid culture condition was temperature 26℃,shaking speed 150 r/min,pH 6.0,15% inoculation amount.