宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究

Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.

以5a生"宁杞1号"为试材,利用分光光度法测定了枸杞果实发育过程中总黄酮的含量变化,采用高效液相色谱法测定了宁夏枸杞果实发育过程中芦丁的含量变化。结果表明:枸杞果实发育过程中总黄酮及其组分芦丁含量总体呈下降趋势。总黄酮含量在花后9d时达到最高,之后随果实的发育逐渐下降;青果期黄酮含量下降较快,至色变期下降趋势减缓,成熟期含量趋于稳定。花后9～19d时,芦丁含量急剧下降,而花后19～37d期间,降至最低点0.012mg/g,其后变化不大,基本趋于稳定。

Taking 5-year-old ＇Ningqi No.1＇ as material,spectrophotometric method was adopted to determine the changes of total flavones content during growth of Lycium barbarum,and HPLC was used to determine the content of rutin.The results showed that the content of flavones and rutin were in decline during the development of Lycium barbarum.The content of flavonoid compounds achieved the highest level when 9 days after blossom,then declined with fruit development.The content of flavones declined more rapidly during green fruit period and declined slowing as colour changes period,at this time,the content of flavones was tending towards stability.The content of rutin fell sharply when 9~19 days after blossom,during the 19~37 days after blossom,reduced to the lowest of 0.012 mg/g,the content of rutin was tending towards stability.