摘要
采用硅胶柱色谱法、薄层色谱法、紫外-可见吸收光谱分析法、核磁共振法、液质联用(HPLC-MS/MS)的多反应监测(MRM)等多种分离与鉴定方法,从人工培养蛹虫草的石油醚、氯仿和甲醇层提取物中分离鉴定出8个化合物,并通过GC-MS技术对人工蛹虫草石油醚提取物进行分析,得出其脂肪酸以亚油酸和反油酸为主,含量分别为41.949%、27.696%。分离得到的8个化合物结构分别被鉴定为:玉米黄素、二氢胆甾醇油酸酯、1-油酰基-2-亚油酸-3-棕榈酸甘油、4,6,8(14),22(23)-四烯-3-酮-麦角甾烷、β-谷甾醇、麦角甾醇过氧化物、啤酒甾醇、甘露醇,其中玉米黄素、二氢胆甾醇油酸酯、1-油酰基-2-亚油酸-3-棕榈酸甘油均是首次从蛹虫草中分离得到。
Eight compounds were isolated from cultured Cordyceps militaris by silica gel column chromatography and recrystallization. Their structures were identified as zeaxanthin, β-cholestanyl oleate, 1-oleoyl-2-1inoleoyl-3-palmitoylglycerol, 4,6,8（14）,22（23）-tetraen-3-one-ergostane, β-sitosterol, ergosterol peroxide, cerevisterol and mannitol by thin layer chromatography （TLC）, HPLC-MS/MS, NMR and El-MS. This study is the first report that zeaxanthin, β-cholestanyl oleate, and 1-oleoyl-2-1inoleoyl-3-palmitoyl glycerol were isolated from Cordyceps militaris, linoleic acid The major fatty acids of petroleum ether extract of cultured Cordyceps militaris were linoleic acid and elaidic acid, acounting for 41.949% and 27.696%, respectively, as analyzed by GC-MS.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第11期36-40,共5页
Food Science
基金
教育部"创新团队发展计划"项目(IRT1134)
