折伦诺对人乳腺癌细胞增殖作用及其类雌激素活性评价

Effect of Zeranol on Proliferation of Human Breast Cancer Cells and Evaluation of Estrogenic Activity

目的:研究折伦诺(Zeranol)对人乳腺癌细胞增殖的影响及评价其类雌激素活性。方法:采用体外细胞培养模型,以MTS/PMS比色法检测Zeranol对雌激素受体阳性乳腺癌细胞系MCF-7和雌激素受体阴性乳腺癌细胞系MDA-MB-231的增殖作用,采用台盼蓝染色和血球计数板计数测定Zeranol作用下MCF-7增殖的细胞生长曲线,并以雌激素受体完全拮抗剂ICI182,780作为工具药物,以此评价Zeranol发挥类雌激素效应与雌激素受体的关系。结果:与对照组相比较,Zeranol(2～50nmol/L)能明显促进MCF-7细胞的增殖,而对MDA-MB-231没有显著影响,同时还发现Zeranol对MCF-7细胞增殖效应能够被雌激素受体拮抗剂ICI182,780完全阻断。结论:低剂量Zeranol能够促进人乳腺癌细胞的增殖,具有类雌激素活性,此作用可能是通过雌激素受体(ER)所介导。

Objective： To explore the effect of zeranol on the proliferation of human breast cancer cells and its estrogenic activity. Methods： in vitro cell culture model was employed and estrogen receptor-positive and estrogen receptor-negative human breast cancer cells were exposed to different doses of Zeranol. The cell proliferation was tested by MTS/PMS assay and the cell growth curve of MCF-7 was measured by trypan blue staining and hemocytometer counting. Moreover, the estrogenic effect of zeranol and its correlation with estrogen receptors were evaluated using estrogen receptor antagonist ICI 182,780 as a tool. Results： Zeranol （2-50 nmol/L） effectively induced the proliferation of MCF-7 cells, but showed no effect on ER-negative MDA-MB-231 cells. Moreover, its effect on MCF-7 cell proliferation was completely blocked by ICI 182,780. Conclusion： Low dose of Zeranol can stimulate the growth of estrogen receptor-positive breast cancer cell and has relatively potent estrogenic activity presumably via estrogen receptor a-mediated pathway.